Table of Parts for an X-OpenSPIM upgrade
This site lists the additional parts required to assemble an X-OpenSPIM configuration (four-lens geometry) with dual sided illumination and dual sided detection as shown in the image below and is an upgrade from the original L-OpenSPIM design.
The described X-OpenSPIM system is equipped with two Andor sCMOS Neo 5.5 camera, which together with the Hamamatsu Flash4 and PCO Edge cameras are known to work with µManager’s Multi-Camera function.
The two laser lines (488 and 561) come from a multiple wavelength laser system Stradus VersaLase and are under the control of an ArduinoUNO board (camera-laser synchronization).
Some self-made parts, including an X-OpenSPIM acquisition chamber, can be purchased from Pieter Fourie Design and Engineering.
Upgrade from L-OpenSPIM to X-OpenSPIM
| Configuration | Accessibility | Description | File or Link/Model | Image | Quantity | Price |
|---|---|---|---|---|---|---|
| X-OpenSPIM | self made | OpenSPIM with four-lens SPIM geometry | X-OpenSPIM.stp (96 MB) For a top view rendering with labelled parts click here. |
 |
1 | ~13000 € (without cameras & self made parts) |
Purchased and self made parts list for the X-OpenSPIM upgrade
| Manufacturer | Accessibility | Description | File or Link/Model | Image | Quantity | Price |
|---|---|---|---|---|---|---|
| Arduino | purchase | Arduino UNO Rev3 SMD | A000073 |  |
1 | 19 € |
| Nikon | purchase | CFI Plan Fluor 10x W/ 0.30/ 3,50 10x water-dipping objective, N.A. 0.10 | CFI Plan Fluor 10X WCFI Plan Fluor 10X W (Thorlabs) |  |
2 | ~1500 € (Thorlabs) |
| Nikon | purchase | CFI Apochromat 40x W NIR/ 0.80/ 3,5040x water-dipping objective, N.A 0.80) | CFI Apochromat NIR 40X WCFI Apochromat NIR 40X W (Thorlabs) |  |
2 | ~2850 € (Thorlabs) |
| PFDE | purchase | Detection axis holder, base | Part 9 |  |
2 | 30 $ |
| PFDE | purchase | Detection axis holder, top | Part 10 |  |
2 | 30 $ |
| Picard Industries | purchase | USB-4D-STAGE R-Axis Upgrade kit | N/A |  |
1 | 185 $ |
| Thorlabs | purchase | 90° Flip Mount for Ø1" Filters and Optics, M4 Tap | TRF90/M |  |
1 | 80,66 € |
| Thorlabs | purchase | Absorptive Neutral Density Filter, SM1-Threaded Mount, ARC: 350-700 nm, OD: 2.0 | NE20A-A |  |
1 | 72,06 € |
| Thorlabs | purchase | Achromatic Doublet, f=19 mm, Ø1/2", SM05-Threaded Mount, ARC: 400-700 nm | AC127-019-A-ML |  |
2 | 77,46 € |
| Thorlabs | purchase | Achromatic Doublet, f=75 mm, Ø1/2", SM05-Threaded Mount, ARC: 400-700 nm | AC127-075-A-ML |  |
2 | 77,46 € |
| Thorlabs | purchase | Adapter for Tube Lens with External SM2 Threads and Internal M38 x 0.5 Threads | SM2A20 |  |
2 | 45,81 € |
| Thorlabs | purchase | Alignment Plate for Ø1" Fixed Lens Mounts | LMR1AP |  |
2 | 23,21 € |
| Thorlabs | purchase | Beamsplitter Cube 50:50 | BS004 |  |
1 | 162,30 € |
| Thorlabs | purchase | Beamsplitter Cube Adapter for Compact 30 mm Cage Cube | BS127CAM |  |
1 | 39,84 € |
| Thorlabs | purchase | Broadband Dielectric Elliptical Mirror | BBE2-E02 |  |
1 | 180,49 € |
| Thorlabs | purchase | Broadband Dielectric Mirror, Ø1", 400 - 750 nm | BB1-E02 |  |
2 | 70,31 € |
| Thorlabs | purchase | Broadband Dielectric Mirror, Ø1/2", 400-750 nm | BB05-E02 |  |
5 | 48,23 € |
| Thorlabs | purchase | Clamping Fork, 1.24" Counterbored Slot, Universal | CF125 |  |
2 | 8,38 € |
| Thorlabs | purchase | Cylindrical Achromat Lens | ACY254-050-A |  |
2 | 369,85 € |
| Thorlabs | purchase | Dovetail Optical Rail, 300 mm, Metric | RLA300/M |  |
2 | 73,54 € |
| Thorlabs | purchase | Gimbal Mirror Mount, Ø25.4 mm | GM100/M |  |
1 | 234,10 € |
| Thorlabs | purchase | ITL200 Tube lens for Nikon | ITL200 |  |
2 | 451,50 € |
| Thorlabs | purchase | Kinematic Mount for Ø12.7 mm Optics, Metric | KM05/M |  |
5 | 36,51 € |
| Thorlabs | purchase | Lens Mount for Ø1/2" Optics, One Retaining Ring Included, M4 Tap | LMR05/M |  |
2 | 14,71 € |
| Thorlabs | purchase | Lens Mount with Retaining Ring for Ø1" Optics, M4 Tap | LMR1/M |  |
2 | 14,26 € |
| Thorlabs | purchase | Mirror 30 mm Cage Cube, M4 Tap | CCM1-4ER/M |  |
1 | 128,86 € |
| Thorlabs | purchase | Pedestal Post Holder | PH20E/M |  |
2 | 22,62 € |
| Thorlabs | purchase | Rotation Mount for Ø1" Optics | RSP1X15/M |  |
1 | 128,86 € |
| Thorlabs | purchase | Right-Angle Kinematic Elliptical Mirror Mount | KCB2EC/M |  |
1 | 221,81 € |
| Thorlabs | purchase | Vacuum-Compatible Optical Post, Ø12.7 mm, M4 Setscrew, M6 Tap, L = 20 mm | TR20V/M |  |
2 | 9,78 € |
| OpenSPIM | self made | 4D-Stage sample arm holder |
4D-Motor-Arm_28mm.stl 4D-Motor-Arm_28mm.stp 4D-Motor-Arm_28mm.pdf |
 |
1 | € |
| OpenSPIM | self made | 4D-Stage syringe holder |
4D-Stage_syringe_holder.stl 4D-Stage_syringe_holder.stp 4D-Stage_syringe_holder.pdf |
 |
1 | € |
| OpenSPIM | self made | Acrylic acquisition chamber |
SampleChamber.stl SampleChamber.stp SampleChamber.pdf |
 |
1 | € |
| OpenSPIM | self made | Dovetail Optical Rail, 300 mm, Metric (cut) | RLA300_M-Step_cut45.stp |  |
2 | € |
| OpenSPIM | self made | Metal holder for acrylic acquisition chamber |
SampleChamberHolder.stl SampleChamberHolder.stp SampleChamberHolder.pdf |
 |
1 | € |
| OpenSPIM | self made | Metal holder bottom for acrylic acquisition chamber |
SampleChamberHolderBottom.stl SampleChamberHolderBottom.stp SampleChamberHolderBottom.pdf |
 |
1 | € |
| OpenSPIM | self made | Modified objective holder ring (part 1) Click here to view a rendering of the assembled objective holder ring (part 1-3. |
ObjectiveHolder_Ring.stl ObjectiveHolder_Ring.stp ObjectiveHolder_Ring.pdf |
 |
4 | € |
| OpenSPIM | self made | Modified objective holder ring (part 2)Handle (piece 1) with rails made of plastic |
Handle_part1.stl Handle_part1-Tail1.stp Handle_part1-Tail1.pdf |
 |
4 | € |
| OpenSPIM | self made | Modified objective holder ring (part 3)Handle (piece 2) |
Handle_part2.stl Handle_part2.stp Handle_part2.pdf |
 |
4 | € |
| OpenSPIM | self made | Infinity space tube |
OpenSPIM_Infinity-Space.stl OpenSPIM_Infinity-Space.stp OpenSPIM_Infinity-Space.pdf |
 |
2 | € |
| OpenSPIM | self made | RC1 Ø1/2" lens stilt |
RC1 Ø1/2" lens stilt.stl RC1 Ø1/2" lens stilt.step RC1 Ø1/2" lens stilt.pdf |
 |
4 | € |
| OpenSPIM | self made | Tube lens adapter to fit new Tube Lens (ITL200), f = 200 mm |
TubeLensAdapter.stl CameraAdapter.stp OpenSPIM_Infinity-Space.pdf CameraAdapterExploded.png |
 |
2 | € |
Purchased and self made parts list for the X-OpenSPIM cooling chamber
| Manufacturer | Accessibility | Description | File or Link/Model | Image | Quantity | Price |
|---|---|---|---|---|---|---|
| Various suppliers |
purchase | Peltier element | TEC1-12706 |  |
1 | 5-25 € |
| OpenSPIM | self made | Cooling Plate The cooling plate is placed between the acrylic chamber and the Peltier element |
CoolingPlate.stl CoolingPlate.step CoolingPlate.pdf |
 |
1 | € |
| OpenSPIM | self made | Heatsink The heatsink has a compartment for the Peltier element |
Heatsink.stl Heatsink.step Heatsink.pdf |
 |
1 | € |
| OpenSPIM | self made | Cooling Walls Part1 These cooling walls face the illumination axis |
CoolingWalls_part1.stl CoolingWalls_part1.step CoolingWalls_part1.pdf |
 |
2 | € |
| OpenSPIM | self made | Cooling Walls Part2 These cooling walls face the detection axis |
CoolingWalls_part2.stl CoolingWalls_part2.step CoolingWalls_part2.pdf |
 |
2 | € |
| OpenSPIM | self made | Teflon Spacer These 1 mm spacers made out of teflon help to thermally insulated the objectives from the heat sink |
TeflonSpacer.stl TeflonSpacer.step TeflonSpacer.pdf |
 |
4 | € |