Table of Parts
All OpenSPIM designed parts need to be double checked for tolerances if you will be 3D printing them!! Having a well trained machinist is an invaluable resource, we recommend that you purchase all of your parts first and then have your OpenSPIM designed parts made to fit afterwards by someone with experience!!
(Pieter Fourie Design and Engineering has made parts for multiple OpenSPIM systems, a link to write an email is beside all self made parts in the list below in the price column)
Illumination Parts List
| Manufacturer | Accessibility | Description | File or Link/Model # | Image | Quantity | Price (EUR) |
|---|---|---|---|---|---|---|
|
Cobolt Coherent Omicron Vortran |
purchase |
Whichever wavelength & mW is necessary for your experiments. Note: the Cobolt MLD 488, Coherent Obis 488 LX, Omicron LuxX488-60, and Vortran Stradus 488-50 packages include the laser unit, control box, interface cable & power supply. Note: For two-color SPIM, the Omicron LightHUB-2 or Vortran VersaLase are available. Modification of the optical layout is required to accommodate the package size. |
Cobolt MLD488 Obis 488 LX LuxX 488-60 Stradus 488-50 LightHUB VersaLase |
 
|
1 | ~7000 |
| OpenSPIM | self made |
The heat sink brings the laser to the desired height of 50 mm off the surface of the optical breadboard Must be made out of a heat conducting material. We use Aluminum. |
Heatsink.stl Heatsink.step Heatsink.pdf |
| 1 | 70, email vendor |
| ThorLabs | purchase |
1/2" (Ø12.7 mm) SM05-Mounted Frosted Glass Alignment Disk w/Ø1 mm Hole Note: for use in aligning the illumination beam path |
DG05-1500-H1-MD | 
| 2 | 25 |
Opto-mechanical parts list
| Manufacturer | Accessibility | Description | File or Link/Model # | Image | Quantity | Price (EUR) |
|---|---|---|---|---|---|---|
| ThorLabs | purchase | The metric (M6 tapped 25 mm spacing) optical bread board that the OpenSPIM system is installed on | MB3045/M | 
| 1 | 185 |
| ThorLabs | purchase | Metric Dovetail Optical Rail, 300 mm plus 2x M6 x 12mm cap screws |
RLA300/M |
| 1 | 65 |
| ThorLabs | purchase | Metric Dovetail Optical Rail, 150 mm plus (at least) 2x M6 x 12mm cap screws for each |
RLA150/M | 
| 2 | 35 |
| ThorLabs | purchase | Metric Dovetail Optical Rail, 75 mm plus 2x M6 x 12mm cap screws |
RLA075/M | 
| 1 | 25 |
| ThorLabs | purchase | Rail carrier 1" (Ø25.4 mm) SD1 Can buy 1 but it wouldn't hurt to buy a 10 pack |
RC1 | 
| 8 | 20 |
| OpenSPIM | self made | The RC1 vertical slit stilt elevates the center of the vertical slit 13.17 mm from the rail carrier to the desired height of 50 mm off the surface of the optical breadboard plus 1x M4 x 22* mm capscrew *cut a M4 x 25 mm down to size |
13.17 mm.stl 13.17 mm.step 13.17 mm.pdf |
| 1 | 35, email vendor |
| ThorLabs | purchase | Adjustable mechanical slit | VA100/M | 
| 1 | 220 |
| OpenSPIM | self made | The RC1 Ø1/2" lens stilt elevates the center of the Ø1/2" (Ø12.7 mm) lenses 14.95 mm from the rail carrier to the desired height of 50 mm off the surface of the optical breadboard plus 1x M4 x 25 mm capscrew for each Note: One could also buy a pack or two of SC1/M's and trim off 0.05 mm, but it might not be such an easy task |
14.95 mm.stl 14.95 mm.step 14.95 mm.pdf |
| 6 | 35, email vendor |
| ThorLabs | purchase | Ø1/2" (Ø12.7 mm) optic mount | LMR05/M | 
| 6 | 15 |
| OpenSPIM | self made | The RC1 Ø1" mirror stilt elevates the center of the Ø1" (Ø25.4 mm) Polaris mirror mounts 5.5 mm from the rail carrier to the desired height of 50 mm off the surface of the optical breadboard plus 1x M4 x 20 mm capscrews and 1x M4 nut for each |
5.5 mm.stl 5.5 mm.step 5.5 mm.pdf |
| 2 | 35, email vendor |
| ThorLabs | modify | Modified Rail carrier 15.4 mm for the cylindrical lens rotation mount Note: made from one of the RC1's |
RC1 mod.pdf | 
| 1 | |
| ThorLabs | purchase | Metric Rotation Mount for Ø1" (Ø25.4 mm) optics, 360° Continuous or 15° Indexed Rotation | RSP1X15/M | 
| 1 | 110 |
| OpenSPIM | self made | The Ø1" mirror stand elevates the center of the Ø1" (Ø25.4 mm) mirror mount 24.55 mm to the desired height of 50 mm off the surface of the optical breadboard plus 1x M4 x 45 mm capscrew and 1x M4 nut |
24.55 mm.stl 24.55 mm.step 24.55 mm.pdf |
| 1 | 35, email vendor |
| ThorLabs | purchase | POLARIS-K1 - PolarisTM Low Drift Ø1" (Ø25.4 mm) Kinematic Mirror Mount Note: it is recommended to use the K1-H for the 2 mirrors closest to the laser |
POLARIS-K1 POLARIS-K1-H |
| 3 | 115 |
Illumination Axis Optical Components List
Opto-mechanical parts list
| Manufacturer | Accessibility | Description | File or Link/Model # | Image | Quantity | Price (EUR) |
|---|---|---|---|---|---|---|
| ThorLabs | purchase | Ø1/2" (Ø12.7 mm) Achromatic Doublet, SM05-Threaded Mount, f = 50 mm, ARC: 400 - 700 nm | AC127-050-A-ML |
 |
2 | 65 |
| ThorLabs | purchase | Ø1/2" (Ø12.7 mm) Achromatic Doublet, SM05-Threaded Mount, f = 25 mm, ARC: 400 - 700 nm | AC127-025-A-ML |  |
2 | 65 |
| ThorLabs | purchase | Ø1" (Ø25.4 mm) Cylindrical Achromat, f = 50 mm, ARC: 350 - 700 nm | ACY254-050-A |  |
1 | 315 |
| Olimpus | purchase | Water dipping objective lens for illumination (10x/0.3) | UMPLFLN 10XW" |  |
1 | 900 |
SPIM Chamber Parts List
| Manufacturer | Accessibility | Description | File or Link/Model # | Image | Quantity | Price (EUR) |
|---|---|---|---|---|---|---|
| OpenSPIM | self made | milled acrylic sample chamber that holds the two Olympus water dipping objectives in a perpendicular arrangement to illuminate and observe the sample plus two M6 x 12mm screws |
Sample Chamber.stl Sample Chamber.step Sample Chamber.pdf |
| 1 | Email vendor, 135 |
| OpenSPIM | self made | metal holder for the acrylic sample chamber (Note:both stl and pdf don't contain dimensions for the M3 holes) | Chamber Holder.stl Chamber Holder.step Chamber Holder.pdf |
| 1 | 200, email vendor |
| OpenSPIM | self made | metal objective holder ring attaching the illumination and detection lens to the metal sample chamber holder. plus three M3 x 8 mm screws for each |
Objective Ring.stl Objective Ring.pdf |
| 2 | 70, email vendor |
| Lelebeck.de | purchase | NBR 70 20x3 mm O-Ring - Creates a water tight seal around the Olympus water dipping objectives | 1164 | 
| 2 | 0.10 |
Detection Axis Optical Components List
| Manufacturer | Accessibility | Description | File or Link/Model # | Image | Quantity | Price (EUR) |
|---|---|---|---|---|---|---|
| Olympus | purchase | Water dipping objective lens for detection (20x/0.5) | UMPLFLN 20XW | 
| 1 | 1650 |
| OpenSPIM | self made | The detection axis holder holds everything behind the detection objective to the camera at the desired height of 50 mm off the surface of the optical breadboard. plus two M5 x 50mm and two M6 x 25mm screws |
Base.stl Base.step Base.pdf Top.stl Top.step Top.pdf |
| 1 | 50, Email vendor |
| OpenSPIM | self made | The infinity space tube defines the distance between the detection objective and its tube lens. plus three M2.5 x 6mm setscrews 120 degrees opposed to each other |
infinity.stl infinity.step infinity.pdf |
| 1 | 70, Email vendor |
| ThorLabs Semrock Chroma Omega |
purchase | Ø1"/25.4 mm Fluorescent Microscopy Filter Make sure to get at least one good bandpass (FWHM 30-50 nm) and a longpass that block the illumination well |
| 100-300 | ||
| OpenSPIM | self made | Ø1"/Ø25.4 mm microscopy fluorescence emission filter holder that fits into OpenSPIM's infinity space Here are some companies who make Ø1" (Ø25.4 mm) microscopy filters: Semrock, Chroma, Omega, and even ThorLabs. Note: the CAD files are showing fancier version compared to the pictures |
Base.stl Base.step Base.pdf Top.stl Top.step Top.pdf |
| 2 | 50, email vendor |
| OpenSPIM | self made | Ø1 1/4" (Ø31.5 mm) astronomy filter holder that fits into OpenSPIM's infinity space Note: the CAD files are showing fancier version compared to the pictures |
Base.stl Base.step Base.pdf Top.stl Top.step Top.pdf |
| 2 | 50, email vendor |
| Olympus | purchase | Tube lens for Olympus detection objective | U-TLU | 
| 1 | 750 |
| Thorlabs (for X-OpenSPIM) | purchase | ITL200 Tube lens for Nikon 40x detection objective (CFI Apochromat NIR 40X W) | ITL200-AutoCADPDF.pdf | 
| 1 | 438,35 |
| X-OpenSPIM | self made | ITL200 tube lens adapter for Nikon 40x detection objective (CFI Apochromat NIR 40X W) | Tube_Lens_adapter_for_Nikon.pdf | 
| 1 | |
| Olympus | purchase | 1x magnifying camera mount | U-TV1 X & U-CMAD3 | 
| 1 | 70 140 |
| Olympus | purchase | 0.5x magnifying camera mount | U-TV0.5XC-3 | 
| 1 | 425 |
4D Motor System Parts List
| Manufacturer | Accessibility | Description | File or Link/Model # | Image | Quantity | Price (EUR) |
|---|---|---|---|---|---|---|
| Picard-Industries | purchase | compact 4 axis motor assembly USB-4D-Stage from Picard-Industries Includes 4 motors assembled on a base plate, sample positioning arm, 5 M3 screws to bolt the sample positioning arm onto the motor assembly |
USB-4D-Stage |  |
1 | 4260 USD, email vendor" |
| OpenSPIM | self made | pulley system to attach to sample holder arm and rotate the sample | sample.stl sample.pdf motor.stl motor.pdf |
| 1 each | |
| any | purchase | O-ring or rubber band for the rotation pulley | any | 
| 1 | |
| OpenSPIM | purchase | Ball bearing system for the front part of the sample rotation pulley system. Note: shown inserted inside the sample positioning arm |
NSK 6001Z | 
| 1 | |
| ThorLabs | purchase | Breadboard clamps to hold the positioned motor assembly onto the breadboard Variable Height Clamp, Metric, M6 Tapped |
CL3/M 2" CL2/M 3" |
| 2-3 | 9 |
Digital Cameras / Electronics
| Manufacturer | Accessibility | Description | File or Link/Model # | Image | Quantity | Price (EUR) |
|---|---|---|---|---|---|---|
| Andor | purchase | Andor Zyla 5.5 or 4.2 | Andor Zyla 5.5 or 4.2 |  |
1 | Ask for quote |
| Hamamatsu | purchase | Hamamatsu ORCA-Flash4.0 LT | Hamamatsu ORCA-Flash4.0 LT |  |
1 | Ask for quote |
| pco | purchase | Pco.edge 4.2 sCMOS | pco.edge 4.2 |  |
1 | Ask for quote |
| Teledyne Photometrics | purchase | Digital cameraKINETIX 10MP | Teledyne Photometrics KINETIX family |  |
1 | Ask for quote |
| Teledyne Photometrics | purchase | Digital cameraPrime BSI Express | Teledyne Photometrics Prime BSI Express |  |
1 | Ask for quote |
| Keyspan | purchase | USB to serial adaptor for the laser | TRIPP-LITE Model-USA-19HS |  |
1 | 50 |
| any electronics vendor | purchase | USB cables for the 4D positioning system and USB hub (at minimum 5 USB positions) |  |
4 & 1 | ||
| any electronics vendor | purchase | USB led lamp for brightfield illumination of the sample during set-up | Conrad electronics |  |
1 | 6 |
Optional Parts List
| Manufacturer | Accessibility | Description | File or Link/Model # | Image | Quantity | Price (EUR) |
|---|---|---|---|---|---|---|
| ThorLabs | purchase | M4 Cap Screw and Hardware Kit | HW-KIT1/M |  |
1 | 50 |
| ThorLabs | purchase | M6 Cap Screw and Hardware Kit | HW-KIT2/M |  |
1 | 95 |
| ThorLabs |
purchase either one of them... |
SM1 Spanner Wrench | SPW602 or SPW606 |  |
1 | 25 |
Once you have all the parts continue on to step by step assembly