Pictures of the OpenSPIM unit and data acquired on it.

OpenSPIM data

Fruit fly

      Histone-YFP from gastrulation to beginning of muscle contraction from ventral, lateral and dorsal view

      Expression pattern of Csp-sGFP recorded with OpenSPIM

      Drosophila blastoderm stage embryo - deconvolved

      Drosophila blastoderm stage embryo - fused using content based fusion

      *Drosophila* gastrulation timelapse 5 views every 6 minutes (HisYFP)

      *Drosophila* gastrulation timelapse 5 views every 5 minutes (HisYFP)

      His-YFP embryo imaged from gastrulation until muscle contractions in late embryogenesis - ventral view

      A slice through a *Drosophila* embryo expressing HisYFP in all cells - unprocessed

Zebrafish

      Multi-position z-stack of a living 48 hpf Tg(Bactin:H2A-EGFP) zebrafish, stitched using the Grid/Collection Plugin in Fiji. Maximum intensity projection, gamma 0.8.

      Multi-position z-stack of a living 48 hpf Tg(Bactin:H2A-EGFP) zebrafish, stitched using the Grid/Collection Plugin in Fiji. Stack video.

      Beating heart of a 48 hpf Tg(cmlc2:EGFP) zebrafish. Left: overlay of transmitted light and fluorescence signal. Right: fluorescence signal.

Volvox

      Z-projection of a stack through a colony of *Volvox carteri*, imaging chlorophyll autofluorescence. The colony is approximately 330 microns wide.

OpenSPIM concept configurations

      1I 1D or L-SPIM

      2I 1D or T-SPIM

      T-SPIM FARM

      2I 2D or X-SPIM

OpenSPIM in a suitcase artsy

      OpenSPIM in a suitcase 1

      OpenSPIM in a suitcase 2

      OpenSPIM in a suitcase 3

      OpenSPIM in a suitcase 4

OpenSPIM with laser on

      Illumination Beam path 1

      Illumination Beam path 2

      Illumination Beam path 3

      Illumination Beam path 4

      Illumination Beam path 5

      Illumination Beam path 6

      Light Sheet in the chamber 1

      Light Sheet in the chamber 2

      Light Sheet in the chamber 3

      Light Sheet in the chamber 4

      Light Sheet in the chamber 5

      Light Sheet in the chamber 6

      Light Sheet in the chamber 7

      Light Sheet in the chamber 8

OpenSPIM with People

      OpenSPIM People MPI-CBG 1

      OpenSPIM People MPI-CBG 2

      OpenSPIM People MPI-CBG 3

      OpenSPIM People MPI-CBG 4

      OpenSPIM People MPI-CBG 5

      OpenSPIM People MPI-CBG 6

      OpenSPIM People MPI-CBG 7

      OpenSPIM People MPI-CBG 8

      OpenSPIM People MPI-CBG 9

      OpenSPIM People MPI-CBG 10

      OpenSPIM People MPI-CBG 11

OpenSPIM Build up videos

      OpenSPIM Solidworks 1

      OpenSPIM Solidworks 1

      OpenSPIM Photo 1

      Open SPIM Photo 2

      OpenSPIM Solidworks and photo

      OpenSPIM Solidworks not looping

      Real buildup stabilized using Preibisch's descriptor-based registration plugin

      Real and Solidworks buildup