Welcome to the X-OpenSPIM site on openspim.org!

Here we provide new instructions and guides on how to assemble, configure, and align an X-OpenSPIM, together with a completely overhauled software plugin μOpenSPIM. This makes it easier and less time-consuming for OpenSPIM users to upgrade to a more advanced OpenSPIM system and use it as a powerful research tool in their laboratories.

• Take a look at the modifications and parts needed to build an X-OpenSPIM
• Follow the alignment guide
• Try the new OpenSPIM plugin (μOpenSPIM) for acquiring data

See also the related publication for detailed instructions on assembly and configuration:

If you find the guides helpful and did something cool with your own X-OpenSPIM please remember to cite us!

Girstmair J., Moon H., Brillard C., Haase R., Tomancak P. (2021) Time to Upgrade: A New OpenSPIM Guide to Build and Operate Advanced OpenSPIM Configurations Advanced Biology
The Supporting Information (PDF) includes details about configuration and the correct alignment of an X-OpenSPIM as well as details about cooling the acquisition chamber.