OpenSPIM for brains setup
The table below lists the parts used by the OpenSPIM for brains setup built by Monika Pawłowska (Nencki Institute of Experimental Biology, Warsaw).
| Manufacturer | Accessibility | Description | File or Link/Model # | Image | Quantity | Price (EUR) |
|---|---|---|---|---|---|---|
| Nikon | purchase | 4X Nikon Plan Fluorite Imaging Objective, 0.13 NA, 17.2 mm WD, dry | N4X-PF |  |
2 (or 3 for double illumination) | 410 |
| Thorlabs | purchase | Translating Lens Mount for Ø1" Optics (for the objectives) | LM1XY/M | 
| 2 or 3 | 125 |
| Nikon | purchase | Infinity-Corrected Tube Lens for Plan Fluorite Objectives | ITL200 |  |
1 | 405 |
| Monika | self made | This chamber can be 3D printed. For windows use 24x24 mm microscopy cover glasses and glue (eg. two component epoxy) | Chamber.stl |  |
1 | |
| Monika | self made | This holder includes two threaded holes so it's easiest to make from metal (eg aluminum). Holder is mounted on the 4D stage arm with M6 screw. Glass slide with 1 mm thickness can be held with nylon or nylon-tipped screw |
Metal_Holder.pdf Metal_Holder.step |
 |
1 |