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This protocol is an important part of sample embedding with FEP tubes, e.g. Zebrafish embryo sample preparation.

material

  • FEP tubes (Bola S1815-04, inner diameter 0.8 mm, outer diameter 1.6 mm)
  • ultrasonicator
  • 1 M NaOH (Merck)
  • 0.5 M NaOH
  • double-distilled H2O
  • 70% ethanol
  • syringe filter (Millex-HV PVDF 0.45 ┬Ám)
  • B. Braun Omnifix F Solo 1 ml Syringe
  • B. Braun needle (100 Sterican, blunt)

File:Fep_cable-drum.JPG|Bola S1815-04 FEP tube File:Syringe_packed.JPG|B. Braun Omnifix F Solo 1 ml Syringe, B. Braun Sterican needle File:Syringe_unpacked.JPG|B. Braun Omnifix F Solo 1 ml Syringe w/ attached needle

procedure

  1. flush tubes with 1 M NaOH, use syringe with attached needle
  2. transfer flushed tubes to fresh Falcon with 0.5 M NaOH, use forceps
  3. put the Falcon in an ultrasonic bath for 10 min
  4. from now on, touch the tubes only with gloves and/or forceps!
  5. transfer the tubes from the Falcon into small basin with ddH2O and flush them with ddH2O
  6. flush the tubes with 70% EtOH
  7. transfer the tubes to a fresh Falcon with 70% EtOH
  8. put the Falcon in an ultrasonic bath for 10 min
  9. transfer the tubes to a fresh Falcon with ddH2O for storage

Category:Operation