The flexibility of light sheet microscopes such as OpenSPIM allows for different configurations. The standard setup consists of one illumination arm ("1I") and one detection arm ("1D"). One can add a second illumination arm ("2I") to improve the homogeneity of the light sheet illumination and/or to reduce the need for rotation. A second detection arm ("2D") enables the simultaneous acquisition from both sides.

basic configurations

	1I 1D	single-sided illumination single-sided detection	xxx / xxx mm (w/d)
	2I 1D	double-sided illumination single-sided detection	xxx / xxx mm (w/d)
	2I 2D	double-sided illumination double-sided detection	xxx / xxx mm (w/d)

high-throughput / multi-sample configurations

FARM

double-sided illumination single-sided detection stack of 4 setups

xxx / xxx / xxx mm (w/d/h)