The flexibility of light sheet microscopes such as OpenSPIM allows for different configurations. The standard setup consists of one illumination arm ("1I") and one detection arm ("1D"). One can add a second illumination arm ("2I") to improve the homogeneity of the light sheet illumination and/or to reduce the need for rotation. A second detection arm ("2D") enables the simultaneous acquisition from both sides.

basic configurations

1I 1D

single-sided illumination
single-sided detection

xxx / xxx mm (w/d)

2I 1D

double-sided illumination
single-sided detection

xxx / xxx mm (w/d)

2I 2D

double-sided illumination
double-sided detection

xxx / xxx mm (w/d)

high-throughput / multi-sample configurations

FARM

double-sided illumination
single-sided detection
stack of 4 setups

xxx / xxx / xxx mm (w/d/h)