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This page is about fluorescent beads, used as fiduciary markers for multi-view registration in light sheet microscopy.

Estapor Fluorescent Microspheres

  • website
  • stock concentration for SPIM: 14 µl 0.5 µm beads in 14 ml medium (sample dependent, e.g. PBS/T for fruit fly, E3 for zebrafish)

tips & tricks

  • extended vortexing and/or treatment in ultrasonic bath is crucial for homogeneous bead distribution and successful bead detection
  • evaluate the bead concentration in agarose w/o specimen

F-XC050

  • diameter 0.5 µm
  • "green" emission (see spectra)
  • EX 488 nm
  • appliations:
    • fiduciary marker in "green" detection channel for bright "green" fluorescence
    • evaluation/adjustment of red-green overlay

F-Y050

  • diameter 0.5 µm
  • "yellow" emission (see spectra)
  • EX 488/561 nm
  • applications:
    • fiduciary marker in "green" detection channel for weak "green" fluorescence
    • fiduciary marker in "red" detection channel for "green" fluorescence

spectral properties

File:Estapor-beads.png|emission spectra