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Step by step assembly

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Disclaimer
When building and working with OpenSPIM it is your own responsibility to make a risk assessment before starting any procedure and on the basis of the risk assessment to make sure appropriate safety precautions are taken to avoid damage to any person or property.

Inform yourself about the manufacturers instructions for all parts used for the openSPIM before you start assembling the openSPIM.

Follow all instructions in the manufacturer’s operator’s manuals, especially the safety precautions.

We take no liability for any personal or property damage caused by the openSPIM or any parts described on this page.

Warning: Depending on the laser class of the laser, the laser beam or even scattered light from the laser may be extremely harmful to eyes and skin. Some lasers can potentially cause blindness after direct eye contact.

Before you start to assemble please make sure you have all needed parts or you know how to improvise them. The light path of our original design is 50 mm off the top surface of the ThorLabs metric optical breadboard (part #: MB3045/M) where everything is installed.

The optical breadboard has a depth of 300 mm and a width of 450 mm, with a grid of 12 x 18 M6 threaded holes spaced 25 mm apart from one another. We will use a coordinate system similar to a spreadsheet (like OpenOffice Calc or Microsoft Excel) to make it easier to know where each component and capscrew is positioned. Starting with A1 in the upper left corner (as seen in the images), and ending with R12 in the lower right.

Please also forgive the confusion about the Youtube videos and the corresponding images, the documentation was done in two different locations months apart from each other and small design changes happened in between.

There is a short introduction to the optics of a SPIM available as the SPIM Optics 101. This will also give practical hints for optics alignment and laser savety.

Assembly overview

OpenSPIM buildup SolidWorks and real
Step Description SolidWorks rendering Real picture Video tutorial
1 Install corner mirror
02a.jpg
Real 02.jpg
Assembly Large-Mirror-Mount to Optical-Breadboard.ogv
2 Install laser heatsink
03a.jpg
Real 03.jpg
Installation Laser-w-Heatsink to Optical-Breadboard Take-2.ogv
3 Install laser
04a.jpg
Real 04.jpg
Installing the laser onto laser base.ogv
4 Installing rails
05a.jpg
Real 05.jpg
Assembly Dovetail-Rails to Optical-Breadboard.ogv
5 Installing laser bouncing mirrors
06a.jpg
Real 06.jpg
Installation Small-Mirror-Assemblies to Dovetail-Rail-System.ogv
6 Installing sample chamber
07a.jpg
Real 07.jpg
Installation SPIM-Chamber-Assembly to Dovetail-Rail-System.ogv
7 Align the laser    
Laser Alignment.ogv
8 Installing beam expander
08a.jpg
Real 08.jpg
Installation Beam Expander.ogv
09a.jpg
Real 09.jpg
9 Installing cylindrical lens
10a.jpg
Real 10.jpg
Installation Cylindrical Lens.ogv
10 Installing vertical slit
11a.jpg
Real 11.jpg
Installation Vertical Slit.ogv
11 Installing the telescope
12a.jpg
Real 12.jpg
Installation Telescope Take-3.ogv
13a.jpg
Real 13.jpg
12 Installing the detection axis
14a.jpg
Real 14.jpg
Installation Detection-Axis Holder-Base.ogv
15a.jpg
Real 15.jpg
Installation Detection-Axis Take-2.ogv
13 Installing the camera
16a.jpg
Real 16.jpg
Installation Detection-Axis Holder-Base.ogv
17a.jpg
Real 17.jpg
14 Installing the 4D motor system
18a.jpg
Real 18.jpg
Installation 4D-Motors.ogv

Now connect the system to the computer and you are ready for operation.