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All OpenSPIM designed parts need to be double checked for tolerances if you will be 3D printing them!! Having a well trained machinist is an invaluable resource, we recommend that you purchase all of your parts first and then have your OpenSPIM designed parts made to fit afterwards by someone with experience!!

Complete reduced.jpg

Illumination Parts List

Manufacturer Accessibility Description File or Link/Model # Image Quantity Price (EUR)
Cobolt/
Coherent/
Omicron/
Vortran
purchase Whichever wavelength & mW is necessary for your experiments.

Note: the Cobolt MLD 488, Coherent Obis 488 LX, Omicron LuxX488-60, and Vortran Stradus 488-50 packages include the laser unit, control box, interface cable & power supply.

Note: For two-color SPIM, the Omicron LightHUB-2 or Vortran VersaLase are available. Modification of the optical layout is required to accommodate the package size.
Cobolt MLD488
Obis 488 LX
LuxX 488-60
Stradus 488-50

LightHUB
VersaLase
Laser cube.jpg

Versa-final-sm.jpg
1 ~7000
OpenSPIM self made The heat sink brings the laser to the desired height of 50 mm off the surface of the optical breadboard

Must be made out of a heat conducting material!! We use Aluminum.
Heatsink.stl
Heatsink.step
Heatsink.pdf
Laser heatsink.jpg
1 70
ThorLabs purchase Ø1/2" (Ø12.7 mm) SM05-Mounted Frosted Glass Alignment Disk w/Ø1 mm Hole

Note: for use in aligning the illumination beam path
DG05-1500-H1-MD
ThorLabs DG05-1500-H1-MD Alignment-Disk.jpg
2 25

Opto-mechanical parts list

Manufacturer Accessibility Description File or Link/Model # Image Quantity Price (EUR)
ThorLabs purchase The metric (M6 tapped 25 mm spacing) optical bread board that the OpenSPIM system is installed on MB3045/M
ThorLabs MB3045-M Aluminum-Breadboard 300x450x12-7mm.jpg
1 185
ThorLabs purchase Metric Dovetail Optical Rail, 300 mm

plus 2x M6 x 12mm cap screws
RLA300/M
ThorLabs RLA075-M Meteric-Dovetail-Optical-Rail Length=300mm.jpg
1 65
ThorLabs purchase Metric Dovetail Optical Rail, 150 mm

plus (at least) 2x M6 x 12mm cap screws for each
RLA150/M
ThorLabs RLA075-M Meteric-Dovetail-Optical-Rail Length=150mm.jpg
2 35
ThorLabs purchase Metric Dovetail Optical Rail, 75 mm

plus 2x M6 x 12mm cap screws
RLA075/M
ThorLabs RLA075-M Meteric-Dovetail-Optical-Rail Length=75mm.jpg
1 25
ThorLabs purchase Rail carrier 1" (Ø25.4 mm)

comes with 1 SD1, but it couldn't hurt to buy one pack of 10 more
RC1
ThorLabs RC1-M Rail-Carrier for Dovetail-Optical-Rail.jpg
8 20
OpenSPIM self made The RC1 vertical slit stilt elevates the center of the vertical slit 13.17 mm from the rail carrier to the desired height of 50 mm off the surface of the optical breadboard

plus 1x M4 x 22* mm capscrew

*cut a M4 x 25 mm down to size
13.17 mm.stl
13.17 mm.step
13.17 mm.pdf
OpenSPIM Stilt Vertical-slit.JPG
1 35
ThorLabs purchase Adjustable mechanical slit VA100/M
ThorLabs VA100-M Adjustable-Mechanical-Slit Metric.jpg
1 220
OpenSPIM self made The RC1 Ø1/2" lens stilt elevates the center of the Ø1/2" (Ø12.7 mm) lenses 14.95 mm from the rail carrier to the desired height of 50 mm off the surface of the optical breadboard

plus 1x M4 x 25 mm capscrew for each

Note: One could also buy a pack or two of SC1/M's and trim off 0.05 mm, but it might not be such an easy task
14.95 mm.stl
14.95 mm.step
14.95 mm.pdf
OpenSPIM Stilt 1-2in-Lens.JPG
6 35
ThorLabs purchase Ø1/2" (Ø12.7 mm) optic mount LMR05/M
ThorLabs LMR05-M Lens-Mount Dia=0-5in.jpg
6 15
OpenSPIM self made The RC1 Ø1" mirror stilt elevates the center of the Ø1" (Ø25.4 mm) Polaris mirror mounts 5.5 mm from the rail carrier to the desired height of 50 mm off the surface of the optical breadboard

plus 1x M4 x 20 mm capscrews and 1x M4 nut for each
5.5 mm.stl
5.5 mm.step
5.5 mm.pdf
OpenSPIM Stilt 1-2in-Mirror.JPG
2 35
ThorLabs modify Modified Rail carrier 15.4 mm for the cylindrical lens rotation mount

Note: made from one of the RC1's
RC1 mod.pdf
MODIFIED ThorLabs RC1-M Rail-Carrier for Dovetail-Optical-Rail-1.jpg
1  
ThorLabs purchase Metric Rotation Mount for Ø1" (Ø25.4 mm) optics, 360° Continuous or 15° Indexed Rotation RSP1X15/M
ThorLabs RSP1X15-M Metric Rotation-Mount Dia=1in.jpg
1 110
OpenSPIM self made The Ø1" mirror stand elevates the center of the Ø1" (Ø25.4 mm) mirror mount 24.55 mm to the desired height of 50 mm off the surface of the optical breadboard

plus 1x M4 x 45 mm capscrew and 1x M4 nut
24.55 mm.stl
24.55 mm.step
24.55 mm.pdf
OpenSPIM Stilt 1in-Mirror.JPG
1 35
ThorLabs purchase POLARIS-K1 - PolarisTM Low Drift Ø1" (Ø25.4 mm) Kinematic Mirror Mount

Note: it is recommended to use the K1-H for the 2 mirrors closest to the laser
POLARIS-K1
POLARIS-K1-H
ThorLabs POLARIS-K1 Kinematic-Mirror-Mount Diameter=1in.jpg
3 115

Illumination Axis Optical Components List

Manufacturer Accessibility Description File or Link/Model # Image Quantity Price (EUR)
ThorLabs purchase Ø1" (Ø25.4 mm) broadband dielectric mirror BB1-E02
ThorLabs BB1-E02 Broadband-Dielectric-Mirror Diameter=1in.jpg
3 65
ThorLabs purchase Ø1/2" (Ø12.7 mm) Achromatic Doublet, SM05-Threaded Mount, f = 50 mm, ARC: 400 - 700 nm AC127-050-A-ML
ThorLabs AC127-050-A-ML Achromatic-Doublet Dia=0-5in F=50mm.jpg
2 65
ThorLabs purchase Ø1/2" (Ø12.7 mm) Achromatic Doublet, SM05-Threaded Mount, f = 25 mm, ARC: 400 - 700 nm AC127-025-A-ML
ThorLabs AC127-025-A-ML Achromatic-Doublet Dia=0-5in F=25mm.jpg
2 65
ThorLabs purchase Ø1" (Ø25.4 mm) Cylindrical Achromat, f = 50 mm, ARC: 350 - 700 nm ACY254-050-A
ThorLabs ACY254-050-A Cylindrical-Achromat Dia=1in f=50mm.jpg
1 315
Olympus purchase Water dipping objective lens for illumination (10x/0.3) UMPLFLN 10XW
Olympus UMPLFLN-10XW 10x-Water-Dippining-Objective-Lens.jpg
1 900

SPIM Chamber Parts List

Manufacturer Accessibility Description File or Link/Model # Image Quantity Price (EUR)
OpenSPIM self made milled acrylic sample chamber that holds the two Olympus water dipping objectives in a perpendicular arrangement to illuminate and observe the sample

plus two M6 x 12mm screws
Sample Chamber.stl
Sample Chamber.step
Sample Chamber.pdf
Sample chamber.jpg
1 135
OpenSPIM self made metal holder for the acrylic sample chamber (Note:both stl and pdf don't contain dimensions for the M3 holes) Chamber Holder.stl
Chamber Holder.step
Chamber Holder.pdf
Sample chamber holder.jpg
1 200
OpenSPIM self made metal objective holder ring attaching the illumination and detection lens to the metal sample chamber holder.

plus three M3 x 8 mm screws for each
Objective Ring.stl
Objective Ring.pdf
Objective ring.jpg
2 70
Lelebeck.de purchase NBR 70 20x3 mm O-Ring - Creates a water tight seal around the Olympus water dipping objectives 1164
O rings.jpg
2 0.10

Detection Axis Optical Components List

Manufacturer Accessibility Description File or Link/Model # Image Quantity Price (EUR)
Olympus purchase Water dipping objective lens for detection (20x/0.5) UMPLFLN 20XW
Olympus UMPLFLN-20XW 20x-Water-Dippining-Objective-Lens.jpg
1 1650
OpenSPIM self made The detection axis holder holds everything behind the detection objective to the camera at the desired height of 50 mm off the surface of the optical breadboard.

plus two M5 x 50mm and two M6 x 25mm screws
Base.stl
Base.step
Base.pdf

Top.stl
Top.step
Top.pdf
Detectionaxis holder.jpg
1 50
OpenSPIM self made The infinity space tube defines the distance between the detection objective and its tube lens.

plus three M2.5 x 6mm setscrews 120 degrees opposed to each other
infinity.stl
infinity.step
infinity.pdf
Infinity space.jpg
1 70
ThorLabs
Semrock
Chroma
Omega
purchase Ø1"/25.4 mm Fluorescent Microscopy Filter

Make sure to get at least one good bandpass (FWHM 30-50 nm) and a longpass that block the illumination well
 
AHF Fluorescent-Filter.jpg
  100-300
OpenSPIM self made Ø1"/Ø25.4 mm microscopy fluorescence emission filter holder that fits into OpenSPIM's infinity space

Here are some companies who make Ø1" (Ø25.4 mm) microscopy filters:
Semrock, Chroma, Omega, and even ThorLabs.

Note: the CAD files are showing fancier version compared to the pictures
Base.stl
Base.step
Base.pdf

Top.stl
Top.step
Top.pdf
Filters holders.jpg
2 50
OpenSPIM self made Ø1 1/4" (Ø31.5 mm) astronomy filter holder that fits into OpenSPIM's infinity space

Note: the CAD files are showing fancier version compared to the pictures
Base.stl
Base.step
Base.pdf

Top.stl
Top.step
Top.pdf
Astro filterholder.jpg
2 50
Olympus purchase Tube lens for detection objective U-TLU (page36)
Olympos tubelenss.jpg
1 750
Olympus purchase 1x magnifying camera mount U-TV1 X & U-CMAD3 (page37)
OneX c-mount.jpg
1 70
140
Olympus purchase 0.5x magnifying camera mount U-TV0.5XC-3 (page 36)
Point5X.jpg
1 425

4D Motor System Parts List

Manufacturer Accessibility Description File or Link/Model # Image Quantity Price (EUR)
Picard-Industries purchase compact 4 axis motor assembly USB-4D-Stage from Picard-Industries

Includes 4 motors assembled on a base plate, sample positioning arm, 5 M3 screws to bolt the sample positioning arm onto the motor assembly
USB-4D-Stage
4d motorsystem.jpg
1 4260 USD
OpenSPIM self made pulley system to attach to sample holder arm and rotate the sample sample.stl
sample.pdf

motor.stl
motor.pdf
Pulleys.jpg
1 each  
any purchase O-ring or rubber band for the rotation pulley any
Assembled motorsystem green top.jpg
1  
OpenSPIM purchase Ball bearing system for the front part of the sample rotation pulley system.

Note: shown inserted inside the sample positioning arm
NSK 6001Z
Ballbearing pulley.jpg
1  
ThorLabs purchase Breadboard clamps to hold the positioned motor assembly onto the breadboard

Variable Height Clamp, Metric, M6 Tapped
CL3/M 2"

CL2/M 3"
Table Clamp.jpg
2-3 9

Electronics

Manufacturer Accessibility Description File or Link/Model # Image Quantity Price (EUR)
Apple purchase Laptop computer to operate the OpenSPIM system. We use Apple MacBook Pro with effectively 4GB of RAM and 256GB SSS hard drive running 32 bit Windows XP.

Note: We are forced to use Windows due to availability of drivers, and the MacBook Pro for it's firewire jack
Mac Book Pro
Computer.jpg
1  
Andor/
Hamamatsu/
pco
purchase
Digital camera



Andor Zyla



Hamamatsu ORCA-Flash4.0 LT



pco.edge 4.2 sCMOS
Andor Zyla 5.5 or 4.2



Hamamatsu ORCA-Flash4.0 LT



pco.edge 4.2
Zyla.png
Flash4 lt.jpg
Pco edge sCMOS.jpg
1 Ask for quote
Keyspan purchase USB to serial adaptor for the laser TRIPP-LITE Model-USA-19HS
Keyspan.jpg
1 50
any electronics vendor purchase USB cables for the 4D positioning system and USB hub (at minimum 5 USB positions)  
Usb cables.jpg
4 & 1  
any electronics vendor purchase USB led lamp for brightfield illumination of the sample during set-up Conrad electronics
Usb led.jpg
1 6

Optional Parts List

Manufacturer Accessibility Description File or Link/Model # Image Quantity Price (EUR)
ThorLabs purchase M4 Cap Screw and Hardware Kit HW-KIT1/M
ThorLabs HW-KIT2-M M6-Capscrew-set.jpg
1 50
ThorLabs purchase M6 Cap Screw and Hardware Kit HW-KIT2/M
ThorLabs HW-KIT1-M M4-Capscrew-set.jpg
1 95
ThorLabs purchase

either one of them...
SM1 Spanner Wrench SPW602 or SPW606
ThorLabs SPW606.jpg
1 25

Once you have all the parts continue on to step by step assembly