Michael Weber from Jan Huisken lab has put together a nice protocol page describing preparation of zebrafish embryos for multi-view SPIM imaging. Lots of photographs!

For more information see the JoVE video of the protocol

• Multilayer Mounting for Long-term Light Sheet Microscopy of Zebrafish

and the paper in Development

• Multilayer mounting enables long-term imaging of zebrafish development in a light sheet microscope