[OpenSPIM] weird images - endian problem?

[Weber, Michael]

Hi Stephanie,

The image looks good to me. I drag-and-dropped it to Fiji, converted the auto-scaled image to 8 bit and saved it as png.

Best,
Michael

[cid:D7B2382C-C94F-4475-87E6-62739BC3A72F at med.harvard.edu]

On Apr 23, 2018, at 1:10 PM, Dr S. Hoehn <S.Hoehn at damtp.cam.ac.uk<mailto:S.Hoehn at damtp.cam.ac.uk>> wrote:


Dear All,

my images/stacks acquired with the OpenSPIM plugin have suddenly started looking weird. I attached an example image (spim_TL00_Angle0.ome.tiff). If I do not define an output directory and save the image manually after acquisition, it looks normal (see attached SimpleOutput.tif).

I have been told it could be a big-endian/little-endian problem (https://en.wikipedia.org/wiki/Endianness).

I haven’t found anything to adjust that turns them back to normal (not contrast/brightness, inversion,…).

Does anyone have an idea how to fix this?

Thank you very much,

Stephanie

<SimpleOutput.tif><spim_TL00_Angle0.ome.tiff>_______________________________________________
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