[OpenSPIM] weird images - endian problem?

Dr S. Hoehn S.Hoehn at damtp.cam.ac.uk
Mon Apr 23 12:13:27 CDT 2018


P.S. The log file reads:

The acquisition is INIT.
Tiles count: 1
calling setBigTiff(true)
Opened log file V:\Users\sh753\2018_04_23_V.ferrisii_test\log.txt
The acquisition is RUNNING.
The acquisition is DONE.




On 2018-04-23 18:10, Dr S. Hoehn wrote:
> Dear All,
> 
> my images/stacks acquired with the OpenSPIM plugin have suddenly
> started looking weird. I attached an example image
> (spim_TL00_Angle0.ome.tiff). If I do not define an output directory
> and save the image manually after acquisition, it looks normal (see
> attached SimpleOutput.tif).
> 
> I have been told it could be a big-endian/little-endian problem
> (https://en.wikipedia.org/wiki/Endianness).
> 
> I haven’t found anything to adjust that turns them back to normal (not
> contrast/brightness, inversion,…).
> 
> Does anyone have an idea how to fix this?
> 
> Thank you very much,
> 
> Stephanie




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