[OpenSPIM] weird images - endian problem?

[Ryan McGorty]

Hi Stephanie,

Yes, it is an endian issue.

You probably want a way to fix this endian problem from happening in the
first place which I don't know how to do. But you can use the Byte Swapper (
https://imagej.nih.gov/ij/plugins/swapper.html) imagej plugin to fix things
after the fact.

Best,
Ryan

On Mon, Apr 23, 2018 at 10:10 AM, Dr S. Hoehn <S.Hoehn at damtp.cam.ac.uk>
wrote:

>
> Dear All,
>
> my images/stacks acquired with the OpenSPIM plugin have suddenly started
> looking weird. I attached an example image (spim_TL00_Angle0.ome.tiff). If
> I do not define an output directory and save the image manually after
> acquisition, it looks normal (see attached SimpleOutput.tif).
>
> I have been told it could be a big-endian/little-endian problem (
> https://en.wikipedia.org/wiki/Endianness).
>
> I haven’t found anything to adjust that turns them back to normal (not
> contrast/brightness, inversion,…).
>
> Does anyone have an idea how to fix this?
>
> Thank you very much,
>
> Stephanie
>
>
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>
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