[OpenSPIM] some bright thing in Fluorescent beads images

[Nikita Vladimirov]

Hi, Yan,

There might be something off with alignment of your system - the 
artefacts are stronger in the left part of the field.
Also, are you using a water-dipping objective with RMSI (RI = 1.457)? If 
the objective is not designed for this refractive index, PSFs will have 
aberrations.
I hope this helps,
Nikita


On 11/10/2017 09:57, Emmanuel Reynaud wrote:
> Dear Yan,
>
> Those "things" seems to be all beyond the beads opposite from the 
> light sheet illumination? If so they maybe illumination artefacts due 
> to the combination of orthogonal laser light and your RMSI. You could 
> easily reduce exposure time by half and/or reduce laser power.
> And to be sure check that your RMSI is clean...
>
> On Wednesday, 11 October 2017, 胡衍 <huyan1020 at sjtu.edu.cn 
> <mailto:huyan1020 at sjtu.edu.cn>> wrote:
> > Hello, everyone.
> >
> > This is Yan.
> > I used our openSPIM to take Fluorescent beads images as shown in the 
> attachment, named figure-original.tif.
> > and I found there are some bright thing along with the beads, as 
> marked by red circles in figure-part.tif.
> >
> > I put the beads in water and water in chamber, then took images, 
> there is no such bright thing in images.
> > But I put the beads in RMIS (RI=1.457), and the same in chamber, 
> then there are such bright thing in images.
> >
> > Is there anyone who knows possible reason for this bright thing in 
> image?
> > What can I do to delete such thing?
> >
> > Thank you very much.
> > Yan
> >
>
> -- 
> *Dr Emmanuel G. Reynaud*
> Principal Investigator, Integrative Biology
> Conway Institute of Biomolecular and Biomedical Science
> UCD Centre for Biomedical Engineering 
> (http://www.ucd.ie/biomedicalengineering/)
>
> e-mail: emmanuel.reynaud at ucd.ie <mailto:matthias.wilm at ucd.ie>
> Website:http://cellbiology.ucd.ie/
>
>
>
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-- 
Nikita Vladimirov, PhD

Preibisch Lab
Max-Delbrück-Centrum
Building 89
Robert-Rössle-Str. 10
13125 Berlin
twitter.com/nvladimus
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