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<p>Hi, Yan,</p>
There might be something off with alignment of your system - the
artefacts are stronger in the left part of the field.<br>
Also, are you using a water-dipping objective with RMSI (RI =
1.457)? If the objective is not designed for this refractive index,
PSFs will have aberrations.<br>
I hope this helps,<br>
Nikita<br>
<br>
<br>
<div class="moz-cite-prefix">On 11/10/2017 09:57, Emmanuel Reynaud
wrote:<br>
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cite="mid:CADdnFiq9PCB=g99=gHOcjmTr=njVce6yV+DvrvYjnXFbcRRj6g@mail.gmail.com">
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Dear Yan,<br>
<br>
Those "things" seems to be all beyond the beads opposite from the
light sheet illumination? If so they maybe illumination artefacts
due to the combination of orthogonal laser light and your RMSI.
You could easily reduce exposure time by half and/or reduce laser
power.<br>
And to be sure check that your RMSI is clean...<br>
<br>
On Wednesday, 11 October 2017, 胡衍 <<a
href="mailto:huyan1020@sjtu.edu.cn" moz-do-not-send="true">huyan1020@sjtu.edu.cn</a>>
wrote:<br>
> Hello, everyone.<br>
><br>
> This is Yan.<br>
> I used our openSPIM to take Fluorescent beads images as shown
in the attachment, named figure-original.tif.<br>
> and I found there are some bright thing along with the beads,
as marked by red circles in figure-part.tif.<br>
><br>
> I put the beads in water and water in chamber, then took
images, there is no such bright thing in images.<br>
> But I put the beads in RMIS (RI=1.457), and the same in
chamber, then there are such bright thing in images.<br>
><br>
> Is there anyone who knows possible reason for this bright
thing in image?<br>
> What can I do to delete such thing?<br>
><br>
> Thank you very much.<br>
> Yan<br>
><br>
<br>
-- <br>
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<div dir="ltr"><b>Dr Emmanuel G.
Reynaud</b><br>
Principal Investigator, Integrative
Biology<br>
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<div dir="ltr">Conway Institute of
Biomolecular and Biomedical Science<br>
<span>UCD Centre for Biomedical
Engineering (<a
href="http://www.ucd.ie/biomedicalengineering/"
target="_blank"
moz-do-not-send="true">http://www.ucd.ie/biomedicalengineering/</a>)</span><cite></cite><br>
<br>
e-mail: <a
href="mailto:matthias.wilm@ucd.ie"
target="_blank"
moz-do-not-send="true">emmanuel.reynaud@ucd.ie</a><br>
Website:<a
href="http://cellbiology.ucd.ie/"
target="_blank"
moz-do-not-send="true">http://cellbiology.ucd.ie/</a><br>
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<pre class="moz-signature" cols="72">--
Nikita Vladimirov, PhD
Preibisch Lab
Max-Delbrück-Centrum
Building 89
Robert-Rössle-Str. 10
13125 Berlin
twitter.com/nvladimus
github.com/nvladimus</pre>
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