[OpenSPIM] some bright thing in Fluorescent beads images

[Emmanuel Reynaud]

Dear Yan,

Those "things" seems to be all beyond the beads opposite from the light
sheet illumination? If so they maybe illumination artefacts due to the
combination of orthogonal laser light and your RMSI. You could easily
reduce exposure time by half and/or reduce laser power.
And to be sure check that your RMSI is clean...

On Wednesday, 11 October 2017, 胡衍 <huyan1020 at sjtu.edu.cn> wrote:
> Hello, everyone.
>
> This is Yan.
> I used our openSPIM to take Fluorescent beads images as shown in the
attachment, named figure-original.tif.
> and I found there are some bright thing along with the beads, as marked
by red circles in figure-part.tif.
>
> I put the beads in water and water in chamber, then took images, there is
no such bright thing in images.
> But I put the beads in RMIS (RI=1.457), and the same in chamber, then
there are such bright thing in images.
>
> Is there anyone who knows possible reason for this bright thing in image?
> What can I do to delete such thing?
>
> Thank you very much.
> Yan
>

-- 
*Dr Emmanuel G. Reynaud*
Principal Investigator, Integrative Biology
Conway Institute of Biomolecular and Biomedical Science
UCD Centre for Biomedical Engineering (
http://www.ucd.ie/biomedicalengineering/)

e-mail: emmanuel.reynaud at ucd.ie <matthias.wilm at ucd.ie>
Website:http://cellbiology.ucd.ie/
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