[OpenSPIM] Cheap Brightfield solution

[Pavel Tomancak]

Hi Michael,

Very nice! Would you mind putting it on the wiki?

All the best

PAvel

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Pavel Tomancak, Ph.D.

Group Leader
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstr. 108
D-01307 Dresden				Tel.: +49 351 210 2670
Germany						Fax: +49 351 210 2020
email: tomancak at mpi-cbg.de
homepage: http://www.mpi-cbg.de/research/research-groups/pavel-tomancak.html
twitter: @PavelTomancak
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On Oct 12, 2015, at 11:28 PM, Michael Redd <mredd at cores.utah.edu> wrote:

> I just put together a cheap solution for a brightfield light for an openSPIM. It uses a 20 cent green diode, an arduino, a 10K potentiometer, 100 Ohm  esistor.  The arduino and light are powered by USB and are turned on and off by micro-manager and can be used as a brightfieqld channel in micromanager multi-dimensional acquisition window. The potentiometer and resistor determine the intensity of the light. The diode is fixed in place behind a collimating lens which fills the back aperture of a 10x immersion lens on axis with the sample and imaging lens.  
>  If your SPIM rig does not have a appropriate port and/or you do not have the 10x lens, one could focus the diode on the sample from above  at an oblique angle. This may require a brighter light, but would probably work with the diode I am using. Light level can be dialed in by using different resistors and potentiometers. 
> I attached photos of the setup and wiring.
> If you have any questions don’t hesitate to email me.
> 
> cheers
> 
> 
> 
> 
> Michael Redd
> Imaging Specialist
> Cell Imaging Core Facility
> University of Utah
> Radiobiology Bldg, Room 48, 
> 30 N 2030 E, Salt Lake City, UT 84112
> 
> michael.redd at hci.utah.edu
> <IMG_0552.jpg><IMG_0556.jpg>
> Michael Redd
> Imaging Specialist
> Cell Imaging Core Facility
> University of Utah
> Radiobiology Bldg, Room 48, 
> 30 N 2030 E, Salt Lake City, UT 84112
> 
> michael.redd at hci.utah.edu
> 
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> OpenSPIM mailing list
> OpenSPIM at openspim.org
> http://openspim.org/mailman/listinfo/openspim

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