<html><head><meta http-equiv="Content-Type" content="text/html charset=windows-1252"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Hi Michael,<div><br></div><div>Very nice! Would you mind putting it on the wiki?</div><div><br></div><div>All the best</div><div><br></div><div>PAvel</div><div><br><div>
<div style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div style="color: rgb(0, 0, 0); font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><div><div style="font-size: 12px; ">-----------------------------------------------------------------------------------<br>Pavel Tomancak, Ph.D.<br><br>Group Leader<br>Max Planck Institute of Molecular Cell Biology and Genetics<br>Pfotenhauerstr. 108<br>D-01307 Dresden<span class="Apple-tab-span" style="white-space: pre; "> </span><span class="Apple-tab-span" style="white-space: pre; "> </span><span class="Apple-tab-span" style="white-space: pre; "> </span><span class="Apple-tab-span" style="white-space: pre; "> </span>Tel.: +49 351 210 2670<br>Germany<span class="Apple-tab-span" style="white-space: pre; "> </span><span class="Apple-tab-span" style="white-space: pre; "> </span><span class="Apple-tab-span" style="white-space: pre; "> </span><span class="Apple-tab-span" style="white-space: pre; "> </span><span class="Apple-tab-span" style="white-space: pre; "> </span><span class="Apple-tab-span" style="white-space: pre; "> </span>Fax: +49 351 210 2020<br>email: <a href="mailto:tomancak@mpi-cbg.de">tomancak@mpi-cbg.de</a><br>homepage: <a href="http://www.mpi-cbg.de/research/research-groups/pavel-tomancak.html">http://www.mpi-cbg.de/research/research-groups/pavel-tomancak.html</a></div><div style="font-size: 12px; ">twitter: @PavelTomancak<br>-----------------------------------------------------------------------------------</div></div><div><br></div></div><br class="Apple-interchange-newline"></div><br class="Apple-interchange-newline"><br class="Apple-interchange-newline">
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<br><div><div>On Oct 12, 2015, at 11:28 PM, Michael Redd <<a href="mailto:mredd@cores.utah.edu">mredd@cores.utah.edu</a>> wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite">
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I just put together a cheap solution for a brightfield light for an openSPIM. It uses a 20 cent green diode, an arduino, a 10K potentiometer, 100 Ohm esistor. The arduino and light are powered by USB and are turned on and off by micro-manager and can be used
as a brightfieqld channel in micromanager multi-dimensional acquisition window. The potentiometer and resistor determine the intensity of the light. The diode is fixed in place behind a collimating lens which fills the back aperture of a 10x immersion lens
on axis with the sample and imaging lens.
<div class=""> If your SPIM rig does not have a appropriate port and/or you do not have the 10x lens, one could focus the diode on the sample from above at an oblique angle. This may require a brighter light, but would probably work with the diode I am using.
Light level can be dialed in by using different resistors and potentiometers. </div>
<div class="">I attached photos of the setup and wiring.</div>
<div class="">If you have any questions don’t hesitate to email me.</div>
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<div class="">cheers</div>
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<div class="">Michael Redd
<div apple-content-edited="true" class="">Imaging Specialist<br class="">
Cell Imaging Core Facility<br class="">
University of Utah<br class="">
Radiobiology Bldg, Room 48, <br class="">
30 N 2030 E, Salt Lake City, UT 84112<br class="">
<br class="">
<a href="mailto:michael.redd@hci.utah.edu" class="">michael.redd@hci.utah.edu</a><br class="">
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<span><IMG_0552.jpg></span><span><IMG_0556.jpg></span><br class="">
<div apple-content-edited="true" class="">Michael Redd<br class="">
Imaging Specialist<br class="">
Cell Imaging Core Facility<br class="">
University of Utah<br class="">
Radiobiology Bldg, Room 48, <br class="">
30 N 2030 E, Salt Lake City, UT 84112<br class="">
<br class="">
<a href="mailto:michael.redd@hci.utah.edu" class="">michael.redd@hci.utah.edu</a><br class="">
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