[OpenSPIM] Cheap Brightfield solution

[Michael Redd]

I just put together a cheap solution for a brightfield light for an openSPIM. It uses a 20 cent green diode, an arduino, a 10K potentiometer, 100 Ohm  esistor.  The arduino and light are powered by USB and are turned on and off by micro-manager and can be used as a brightfieqld channel in micromanager multi-dimensional acquisition window. The potentiometer and resistor determine the intensity of the light. The diode is fixed in place behind a collimating lens which fills the back aperture of a 10x immersion lens on axis with the sample and imaging lens.
 If your SPIM rig does not have a appropriate port and/or you do not have the 10x lens, one could focus the diode on the sample from above  at an oblique angle. This may require a brighter light, but would probably work with the diode I am using. Light level can be dialed in by using different resistors and potentiometers.
I attached photos of the setup and wiring.
If you have any questions don’t hesitate to email me.

cheers




Michael Redd
Imaging Specialist
Cell Imaging Core Facility
University of Utah
Radiobiology Bldg, Room 48,
30 N 2030 E, Salt Lake City, UT 84112

michael.redd at hci.utah.edu<mailto:michael.redd at hci.utah.edu>
[cid:24D312A2-F29D-437F-8526-CF48F1D269C2 at cores.utah.edu][cid:CE1AB7A6-65AD-42EE-B2AE-6E416842776C at cores.utah.edu]
Michael Redd
Imaging Specialist
Cell Imaging Core Facility
University of Utah
Radiobiology Bldg, Room 48,
30 N 2030 E, Salt Lake City, UT 84112

michael.redd at hci.utah.edu<mailto:michael.redd at hci.utah.edu>

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