[OpenSPIM] Cheap Brightfield solution

[Michael Weber]

A first version is now in the wiki:

 

http://openspim.org/Brightfield

 

Feel free to add your brightfield solutions!

 

Michael

 

From: openspim-bounces at openspim.org [mailto:openspim-bounces at openspim.org]
On Behalf Of Pavel Tomancak
Sent: Tuesday, October 13, 2015 8:28 AM
To: Michael Redd <mredd at cores.utah.edu>
Cc: openspim at openspim.org
Subject: Re: [OpenSPIM] Cheap Brightfield solution

 

Hi Michael,

 

Very nice! Would you mind putting it on the wiki?

 

All the best

 

PAvel

 

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Pavel Tomancak, Ph.D.

Group Leader
Max Planck Institute of Molecular Cell Biology and Genetics
Pfotenhauerstr. 108
D-01307 Dresden                                              Tel.: +49 351
210 2670
Germany
Fax: +49 351 210 2020
email: tomancak at mpi-cbg.de <mailto:tomancak at mpi-cbg.de> 
homepage: http://www.mpi-cbg.de/research/research-groups/pavel-tomancak.html

twitter: @PavelTomancak
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On Oct 12, 2015, at 11:28 PM, Michael Redd <mredd at cores.utah.edu
<mailto:mredd at cores.utah.edu> > wrote:





I just put together a cheap solution for a brightfield light for an
openSPIM. It uses a 20 cent green diode, an arduino, a 10K potentiometer,
100 Ohm  esistor.  The arduino and light are powered by USB and are turned
on and off by micro-manager and can be used as a brightfieqld channel in
micromanager multi-dimensional acquisition window. The potentiometer and
resistor determine the intensity of the light. The diode is fixed in place
behind a collimating lens which fills the back aperture of a 10x immersion
lens on axis with the sample and imaging lens.   

 If your SPIM rig does not have a appropriate port and/or you do not have
the 10x lens, one could focus the diode on the sample from above  at an
oblique angle. This may require a brighter light, but would probably work
with the diode I am using. Light level can be dialed in by using different
resistors and potentiometers. 

I attached photos of the setup and wiring.

If you have any questions don't hesitate to email me.

 

cheers

 

 

 

 

Michael Redd 

Imaging Specialist
Cell Imaging Core Facility
University of Utah
Radiobiology Bldg, Room 48, 
30 N 2030 E, Salt Lake City, UT 84112

michael.redd at hci.utah.edu <mailto:michael.redd at hci.utah.edu> 

 

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