[OpenSPIM] holographic diffusers

[Feinstein, Timothy N]

Hi folks,

Salili and co-workers (Review Sci. Instruments 89: 036107) just published a very interesting technique for eliminating stripe artifacts using elliptical holographic diffusers.  It looks like a much simpler version of mSPIM, with just a couple filters and no moving parts.  This would be great news for me since my mSPIM design doesn’t work – instead of tilting, the sheet moves up and down in a parallel track.

Has anyone tried holographic diffusers before?  They look like they need to be quite close to the sample, so it’s possible they would not work with a conventional openSPIM sample holder.  Any feedback would be appreciated.

Thanks!


Tim

Timothy Feinstein, Ph.D.
Research Scientist
Department of Developmental Biology
University of Pittsburgh


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