[OpenSPIM] holographic diffusers

[Jan Huisken]

Hi Tim

We have not yet tried those diffusers.

Why is your mSPIM not working? Sounds like your resonant mirror is not in the right plane. Please let us know if there is anything we can help with.

Best
Jan

> On Apr 9, 2018, at 8:50 AM, Feinstein, Timothy N <tnf8 at PITT.EDU> wrote:
> 
> Hi folks, 
>  
> Salili and co-workers (Review Sci. Instruments 89: 036107) just published a very interesting technique for eliminating stripe artifacts using elliptical holographic diffusers.  It looks like a much simpler version of mSPIM, with just a couple filters and no moving parts.  This would be great news for me since my mSPIM design doesn’t work – instead of tilting, the sheet moves up and down in a parallel track. 
>  
> Has anyone tried holographic diffusers before?  They look like they need to be quite close to the sample, so it’s possible they would not work with a conventional openSPIM sample holder.  Any feedback would be appreciated.  
>  
> Thanks!
>  
> 
> Tim
>  
> Timothy Feinstein, Ph.D. 
> Research Scientist
> Department of Developmental Biology
> University of Pittsburgh
>  
>  
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