[OpenSPIM] some bright thing in Fluorescent beads images

胡衍 huyan1020 at sjtu.edu.cn
Wed Oct 11 02:40:03 CDT 2017


Hello, everyone.

This is Yan.
I used our openSPIM to take Fluorescent beads images as shown in the attachment, named figure-original.tif.
and I found there are some bright thing along with the beads, as marked by red circles in figure-part.tif.

I put the beads in water and water in chamber, then took images, there is no such bright thing in images.
But I put the beads in RMIS (RI=1.457), and the same in chamber, then there are such bright thing in images.

Is there anyone who knows possible reason for this bright thing in image? 
What can I do to delete such thing?

Thank you very much.
Yan
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