[OpenSPIM] no structure in brain tissue image

[Stephan.Preibisch at mdc-berlin.de]

Hi, just a few questions which may help:

- are you sure your light sheet is aligned well?
- are you imaging too deep inside the sample?
- did the clearing actually work well, it almost looks like it is just not well cleared?

All the best, hope it helps,
Stephan

---

Dr. Stephan Preibisch
Group Leader

Berlin Institute for Medical Systems Biology (BIMSB), Max Delbrück Center for Molecular Medicine (MDC)
Building 89, 1.08b
Robert-Rössle-Str. 10
13125 Berlin

email: stephan.preibisch at mdc-berlin.de<mailto:stephan.preibisch at mdc-berlin.de>
web: http://preibischlab.mdc-berlin.de
twitter: http://twitter.com/preibischs

On Nov 20, 2017, at 7:06 PM, 胡衍 <huyan1020 at sjtu.edu.cn<mailto:huyan1020 at sjtu.edu.cn>> wrote:

Hello, everyone.

This is Yan.

I used my openSPIM to take images for CLARITY brain tissue,
and the results as shown in the attachment.
I cannot find any structure in these images.

Is there anyone knowing such condition?
What can I do to improve this condition?

I am looking forward for your reply.
Best regards,
Yan<img_13.tif><img_14.tif><img_15.tif>_______________________________________________
OpenSPIM mailing list
OpenSPIM at openspim.org<mailto:OpenSPIM at openspim.org>
http://openspim.org/mailman/listinfo/openspim

-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://openspim.org/pipermail/openspim/attachments/20171121/547075ea/attachment.html>