[OpenSPIM] thicker light sheet

[Weber, Michael]

Hi Monika,

Using a slit is a quick and flexible solution. By reducing the magnification of the illumination objective you’re also increasing the light sheet height, losing laser power but gaining illumination uniformity for a given field of view. You’d need to modify the ratio of beam expander and telescope to modify the light sheet properties. You can find examples for calculating the optics for light sheet illumination in a recent book chapter:

http://www.wiley.com/WileyCDA/WileyTitle/productCd-3527338373.html

Best,
Michael

On Jun 5, 2017, at 8:42 AM, Monika Pawłowska <m.pawlowska at nencki.gov.pl<mailto:m.pawlowska at nencki.gov.pl>> wrote:

Dear all,

just to make sure that my understanding of optics is not failing me: if I want to have a thicker (and more uniform) light sheet, but with the same height (same FOV), I can
-use a slit, losing power and introducing diffraction pattern
-exchange illumination objective into lower magnification (losing money ;) )

are there other options?

Regards,

--
Dr Monika Pawłowska
Nencki Institute
02-093 Warsaw
Pasteura 3
Poland

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_____________

Dr. Michael Weber
Advanced Microscopy Fellow
Harvard Medical School
240 Longwood Ave, LHRRB 113, Boston, MA 02115
http://nic.med.harvard.edu

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