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<div class="">Hi Monika,</div>
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<div class="">Using a slit is a quick and flexible solution. By reducing the magnification of the illumination objective you’re also increasing the light sheet height, losing laser power but gaining illumination uniformity for a given field of view. You’d need
to modify the ratio of beam expander and telescope to modify the light sheet properties. You can find examples for calculating the optics for light sheet illumination in a recent book chapter:</div>
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<div class=""><a href="http://www.wiley.com/WileyCDA/WileyTitle/productCd-3527338373.html" class="">http://www.wiley.com/WileyCDA/WileyTitle/productCd-3527338373.html</a></div>
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<div class="">Best,</div>
<div class="">Michael</div>
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<div class="">On Jun 5, 2017, at 8:42 AM, Monika Pawłowska <<a href="mailto:m.pawlowska@nencki.gov.pl" class="">m.pawlowska@nencki.gov.pl</a>> wrote:</div>
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<div class="">Dear all,<br class="">
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just to make sure that my understanding of optics is not failing me: if I want to have a thicker (and more uniform) light sheet, but with the same height (same FOV), I can<br class="">
-use a slit, losing power and introducing diffraction pattern<br class="">
-exchange illumination objective into lower magnification (losing money ;) )<br class="">
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are there other options?<br class="">
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Regards,<br class="">
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-- <br class="">
Dr Monika Pawłowska<br class="">
Nencki Institute<br class="">
02-093 Warsaw<br class="">
Pasteura 3<br class="">
Poland<br class="">
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<div class=""><span lang="EN" class="">_____________<br class="">
<br class="">
Dr. Michael Weber<br class="">
Advanced Microscopy Fellow<br class="">
Harvard Medical School<br class="">
240 Longwood Ave, LHRRB 113, </span>Boston, MA 02115</div>
<div class=""><a href="http://nic.med.harvard.edu" class="">http://nic.med.harvard.edu</a></div>
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