[OpenSPIM] which C-mount adapter

[Craig Russell]

> I wanted to ask why there is a choice of two different C-mount
> adapters, one is 1x and the other 0.5x magnifying?

The 0.5x will increase the FOV.

> Does this have anything to do with matching the microscope resolution
> with the pixel size of the used camera?

To maximise your resolution without having "empty magnification" you 
would need to ensure that for a single PSF you have roughly 4 square 
pixels to image it with. Anything less and you will be under sampling 
your image, too many more and you'll be distributing unnecessary shot 
noise into your image.

For the magnifications and NAs that the openSPIM uses the nyquist 
criterion is likely never reached or even needed.

Best

Craig

On 2017-07-20 12:54, Dr S. Hoehn wrote:
> Dear All,
> 
> I wanted to ask why there is a choice of two different C-mount
> adapters, one is 1x and the other 0.5x magnifying?
> 
> Does this have anything to do with matching the microscope resolution
> with the pixel size of the used camera?
> 
> Or is the 0.5x magnifying one used to increase the field of view?
> 
> Thanks a lot.
> 
> Best wishes,
> Stephanie
> 
> -----------------------------------
> 
> Dr. Stephanie Hoehn
> DAMTP, Biological Physics
> Centre for Mathematical Sciences
> Wilberforce Road
> Cambridge
> CB3 0WA
> United Kingdom
> Office Tel: 01223 337859
> Office: H0.03
> 
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-- 
Craig Russell, PhD Candidate
Dept. Chemical Engineering and Biotechnology, Cambridge
+44 (0)7521650931 | laser.ceb.cam.ac.uk