[OpenSPIM] which C-mount adapter

[Jan Huisken]

Dear Stephanie

The Olympus lenses have a field number of 26.5 mm. This is the diameter of the (circular) image that you get after the tubelens and that you try to capture with your camera. It thereby defines the maximally achievable field of view, e.g. for a 10x lens it is 26.5/10 = 2.65mm. If your camera chip, however, is smaller, then you will not capture the full field of view. For example a Zyla 5.5 sCMOS camera has a diagonal of 21.8mm, which is pretty big but not big enough. The 0.5x adapter will demagnify the image to 26.5/2=13.25mm. It is specifically designed for 1/2in CCD chips since these have a diagonal of 25.4/2=12.7mm, which fits well into the smaller image. It is important to understand that the adapter does not increase the field of view; it only makes the image smaller and adapts it to your chip. You cannot use the 0.5x adapter with an sCMOS camera, e.g. to get twice the field of view.

Hope this helps
Jan

> On Jul 20, 2017, at 6:54 AM, Dr S. Hoehn <S.Hoehn at damtp.cam.ac.uk> wrote:
> 
> Dear All,
> 
> I wanted to ask why there is a choice of two different C-mount adapters, one is 1x and the other 0.5x magnifying?
> 
> Does this have anything to do with matching the microscope resolution with the pixel size of the used camera?
> 
> Or is the 0.5x magnifying one used to increase the field of view?
> 
> Thanks a lot.
> 
> Best wishes,
> Stephanie
> 
> -----------------------------------
> 
> Dr. Stephanie Hoehn
> DAMTP, Biological Physics
> Centre for Mathematical Sciences
> Wilberforce Road
> Cambridge
> CB3 0WA
> United Kingdom
> Office Tel: 01223 337859
> Office: H0.03
> 
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