[OpenSPIM] High background in the light sheet microscopy images

[Craig Russell]

You're also saturating your image with your beads, feel free to reduce
the power/exposure time.

Craig Russell, PhD Candidate
Dept. Chemical Engineering and Biotechnology, Cambridge
+44 (0)7521650931 | laser.ceb.cam.ac.uk <http://laser.ceb.cam.ac.uk/>
Weber, Michael:
>
> Hi Yan,
>
>  
>
> The beads in the left part of your image look suspicious, are you
> illuminating and/or detecting through the cuvette?
>
>  
>
> Best,
>
> Michael
>
>  
>
> *From: *胡衍 <mailto:huyan1020 at sjtu.edu.cn>
> *Sent: *Wednesday, January 11, 2017 8:46 PM
> *To: *openspim at openspim.org <mailto:openspim at openspim.org>
> *Subject: *[OpenSPIM] High background in the light sheet microscopy images
>
>  
>
> Hello, everyone.
>
> I built a light sheet microscopy followed as the openSPIM.
> But I got the images with high background as shown in the attachment.
> The exposure time is 80ms.
> The fluorescent beads, which are embedded in 6% PAGE gel, are in a
> cuvette.
>
> Will anyone know the reason for such high background?
> What can I do to reduce the background?
>
> Thank you so much.
>
> Best regards,
> Yan
>
>
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