[OpenSPIM] High background in the light sheet microscopy images

[Weber, Michael]

Hi Yan,



The beads in the left part of your image look suspicious, are you illuminating and/or detecting through the cuvette?



Best,

Michael



From: 胡衍<mailto:huyan1020 at sjtu.edu.cn>
Sent: Wednesday, January 11, 2017 8:46 PM
To: openspim at openspim.org<mailto:openspim at openspim.org>
Subject: [OpenSPIM] High background in the light sheet microscopy images



Hello, everyone.

I built a light sheet microscopy followed as the openSPIM.
But I got the images with high background as shown in the attachment.
The exposure time is 80ms.
The fluorescent beads, which are embedded in 6% PAGE gel, are in a cuvette.

Will anyone know the reason for such high background?
What can I do to reduce the background?

Thank you so much.

Best regards,
Yan
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