[OpenSPIM] variable PSF across light sheet

Monika Pawłowska m.pawlowska at nencki.gov.pl
Fri Jan 20 07:34:49 CST 2017


Hi all,

maybe somebody know a paper on dealing with variable PSF across the image,  
resulting from the changing light-sheet thickness (i.e. light sheet  
getting thicker further away from the centre)? For bigger samples, as the  
ones I image, keeping the light sheet thickness uniform would result in a  
very thick light sheet, so I was wondering if there is another method,  
given that PSF changes in a predictable way.

Best regards,

-- 
Dr Monika Pawłowska
Nencki Institute
02-093 Warsaw
Pasteura 3
Poland



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