[OpenSPIM] light sheet characterization in solvents (BABB etc)

[Monika Pawłowska]

Hi Claire!

Thanks for the tip, but actually I already tried the glass. However, the  
double reflection from both surfaces makes automatic analysis (ie fitting  
curves to extract width) more difficult and besides I am getting some  
weirdness in the results, so I was wondering if it can be caused by the  
glass being uneven or something like that.

Regards,
Monika

W dniu .04.2017 o 11:10 Claire Lionnet <claire.lionnet at ens-lyon.fr> pisze:

> Hi Monika,
>
> Le 03/04/2017 à 11:59, Monika Pawłowska a écrit :
>> Hi,
>>
>> I know that most people here use samples in water, but I don't know  
>> where else to ask, so:
>>
>> have you ever tried to characterize the light sheet thickness not in  
>> water, but BABB (or other solvent)? Will it damage a silver mirror? Is  
>> there any other way?
> I don't use cleared tissue myself, and I don't know what BABB could do  
> to a silver mirror, but you could simply use a glass coverslip instead :  
> it requires more laser power/less attenuation than with a mirror, but  
> the reflection of the lightsheet is strong enough. And it's cheaper !
>
> Cheers,
>
> Claire
>
>


-- 
Dr Monika Pawłowska
Nencki Institute
02-093 Warsaw
Pasteura 3
Poland