[OpenSPIM] light sheet characterization in solvents (BABB etc)

[Claire Lionnet]

Hi Monika,

Le 03/04/2017 à 11:59, Monika Pawłowska a écrit :
> Hi,
>
> I know that most people here use samples in water, but I don't know 
> where else to ask, so:
>
> have you ever tried to characterize the light sheet thickness not in 
> water, but BABB (or other solvent)? Will it damage a silver mirror? Is 
> there any other way?
I don't use cleared tissue myself, and I don't know what BABB could do 
to a silver mirror, but you could simply use a glass coverslip instead : 
it requires more laser power/less attenuation than with a mirror, but 
the reflection of the lightsheet is strong enough. And it's cheaper !

Cheers,

Claire


-- 
Claire LIONNET
PLATIM
US8 / UMS3444 BioSciences Gerland - Lyon Sud
http://www.sfr-biosciences.fr/plateformes/Phenotypage-cellulaire/PLATIM