[OpenSPIM] light sheet characterization in solvents (BABB etc)

[Monika Pawłowska]

Hi,

I know that most people here use samples in water, but I don't know where  
else to ask, so:

have you ever tried to characterize the light sheet thickness not in  
water, but BABB (or other solvent)? Will it damage a silver mirror? Is  
there any other way?

We prepared an agarose sample with fluorescent beads and dehydrated it  
like in this paper:  
https://www.jove.com/video/50696/micron-scale-resolution-optical-tomography-entire-mouse-brains-with

and the agarose stayed in one piece, but there is very little signal, so  
I'm afraid that the beads might have dissolved.

Regards,

-- 
Dr Monika Pawłowska
Nencki Institute
02-093 Warsaw
Pasteura 3
Poland