[OpenSPIM] Optical abberations

[Nikita Vladimirov]

Hi, Matt,
If you have to choose between these two lenses, I would definitely go with
the achromat. I would actually recommend 2" diameter lens (f=200 mm),
because the quality of surface is best at the lens center, and 1" lens is
quite small for your purposes.
 Also, at a price of $100 this lens is not a big saving compared to the 200
mm standard tube lens for Nikon objectives (you can buy it from Nikon for
$250 I think).
Best,
Nikita



On Thu, Nov 17, 2016 at 6:05 AM, FORD Matthew <Matthew.Ford at ed.ac.uk> wrote:

> Thanks for your advice. We are using either leica or nikon objectives for
> detection so I think a f=200mm is needed. I have found two lens on thorlabs
> which apear to do the job.
>
>
> https://www.thorlabs.com/thorproduct.cfm?partnumber=LBF254-200-A
>
>
> https://www.thorlabs.com/thorproduct.cfm?partnumber=AC254-200-A-ML
>
>
> One is an achromat doublet while the other is spherical. Do you have any
> advice on which would perform better as a tube lens using mutiple lazer
> lines?
>
>
> Best wishes,
>
>
> Matt
> ------------------------------
> *From:* Nikita Vladimirov <nvladimus at gmail.com>
> *Sent:* 17 November 2016 00:53:14
> *To:* FORD Matthew
> *Cc:* openspim at openspim.org
> *Subject:* Re: [OpenSPIM] Optical abberations
>
> Hi, Matt,
> The image seems to have really strong astigmatism, and the optical axis is
> way off center. The lens you use has f=25mm, it is a far cry from what the
> tube lens is expected to be, f=180 mm for Olympus objective. You cannot
> expect any decent quality from a tube lens which is so far from what the
> system design (objective - tube lens). You need a proper tube lens, or at
> least an achromat with similar focal distance to have a decent image
> quality.
> Best,
> Nikita
>
>
>
> On Wed, Nov 16, 2016 at 11:52 AM, FORD Matthew <Matthew.Ford at ed.ac.uk>
> wrote:
>
>> Hi OpenSPIMers,
>>
>>
>> I am in the process of building an open spim system based on the original
>> open spim. I've tried to image some fluorescent beads but am seeing an odd
>> effect where beads around the edge are being distored with the curvature
>> of the lens (I have attached an image). We are using a * LA1951-A -
>> N-BK7 Plano-Convex Lens* as a tube lens rather than the olympus U-TLU. I
>> was wondering if the tube lens is what is causing the abborations but I am
>> fairly new to optics so am not sure. If anyone has any advice on what might
>> be causing the abborations I would lvoe to hear your suggestions as I'm a
>> bit stuck.
>>
>>
>> Thanks in advance for your advice,
>>
>>
>> Matt
>>
>> The University of Edinburgh is a charitable body, registered in
>> Scotland, with registration number SC005336.
>>
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>>
>
> The University of Edinburgh is a charitable body, registered in
> Scotland, with registration number SC005336.
>
>
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