[OpenSPIM] Optical abberations

[Monika Pawłowska]

I agree with Nikita, but independently from that, if the objective is  
infinity-corrected, it is possible to test a tube lens and its distance  
 from camera by attaching a tube lens to the camera, taking it to a big  
room and trying to get a sharp image of something at the other end of the  
room (I was taking a photo of a reagent bottle trying to get the text on  
the label sharp...)

Regards,
Monika

W dniu .11.2016 o 01:53 Nikita Vladimirov <nvladimus at gmail.com> pisze:

> Hi, Matt,
> The image seems to have really strong astigmatism, and the optical axis  
> is way off center. The lens you use has f=25mm, it is a far cry from  
> what the >tube lens is expected to be, f=180 mm for Olympus objective.  
> You cannot expect any decent quality from a tube lens which is so far  
> from what the >system design (objective - tube lens). You need a proper  
> tube lens, or at least an achromat with similar focal distance to have a  
> decent image quality.Best,
> Nikita
>
>
>
> On Wed, Nov 16, 2016 at 11:52 AM, FORD Matthew <Matthew.Ford at ed.ac.uk>  
> wrote:
>>
>> Hi OpenSPIMers,
>>
>>
>>
>> I am in the process of building an open spim system based on the  
>> original open spim. I've tried to image some >>fluorescent beads but am  
>> seeing an odd effect where beads around the edge are being distored  
>> with the curvature  of >>the lens (I have attached an image). We are  
>> using a LA1951-A - N-BK7 Plano-Convex Lens as a tube lens rather than  
>> >>the olympus U-TLU. I was wondering if the tube lens is what is  
>> causing the abborations but I am fairly new to optics so >>am not sure.  
>> If anyone has any advice on what might be causing the abborations I  
>> would lvoe to hear your suggestions >>as I'm a bit stuck.
>>
>>
>>
>> Thanks in advance for your advice,
>>
>>
>>
>> Matt
>> The University of Edinburgh is a charitable body, registered in
>> Scotland, with registration number SC005336.
>>
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>



-- 
Dr Monika Pawłowska
Nencki Institute
02-093 Warsaw
Pasteura 3
Poland
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