[OpenSPIM] general questions for OpenSpim

[Ly, Sonny S.]

Hi OpenSpim Community,

We at LLNL am interested in developing light sheet microscopy for host-pathogen interactions.
We would like to do cellular imaging and not so much tissue or embryonic imaging.
Would the OpenSpim setup be suitable for this?  In addition, we have some other questions:

1.       What is the highest N.A. objective we can use?
2.       Can we incorporate Bessel beam, or even lattice light sheet like Eric Betzig's group?
3.       Are there an advantages of using stepper motors for scanning, and not galvo-mirrors?  Can galvos be used instead?

Thank you.


Sonny Ly
Staff Scientist
Lawrence Livermore National Laboratory

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