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<p class="MsoNormal">Hi OpenSpim Community,<o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
<p class="MsoNormal">We at LLNL am interested in developing light sheet microscopy for host-pathogen interactions.
<o:p></o:p></p>
<p class="MsoNormal">We would like to do cellular imaging and not so much tissue or embryonic imaging.
<o:p></o:p></p>
<p class="MsoNormal">Would the OpenSpim setup be suitable for this? In addition, we have some other questions:<o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
<p class="MsoNormal">1. What is the highest N.A. objective we can use?<o:p></o:p></p>
<p class="MsoNormal">2. Can we incorporate Bessel beam, or even lattice light sheet like Eric Betzig’s group?
<o:p></o:p></p>
<p class="MsoNormal">3. Are there an advantages of using stepper motors for scanning, and not galvo-mirrors? Can galvos be used instead?<o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
<p class="MsoNormal">Thank you.<o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
<p class="MsoNormal"> <o:p></o:p></p>
<p class="MsoNormal">Sonny Ly<o:p></o:p></p>
<p class="MsoNormal">Staff Scientist<o:p></o:p></p>
<p class="MsoNormal">Lawrence Livermore National Laboratory<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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