[OpenSPIM] general questions for OpenSpim ** COMMERCIAL REPLY **

[Jon]

Hi Sonny,

I suspect the key challenge will be mounting your cells in the OpenSPIM 
geometry.  But there are light sheet microscopes built around a 
horizontal sample mount, including diSPIM, lattice light sheet, and 
Leica's confocal add-on.

For the diSPIM (http://dispim.org/) it is possible to use a NA 1.0 or 
1.1 objective if the usual 0.8 NA isn't enough.  It's also possible to 
incorporate Bessel beam, etc.  I'm happy to answer further questions but 
we should move off this mailing list.

Jon

------------------------------------------
Jon Daniels
Applied Scientific Instrumentation
29391 West Enid Rd, Eugene, OR 97402
Phone: (541) 461-8181 x118
------------------------------------------


> Hi OpenSpim Community,
>
> We at LLNL am interested in developing light sheet microscopy for
> host-pathogen interactions.
>
> We would like to do cellular imaging and not so much tissue or embryonic
> imaging.
>
> Would the OpenSpim setup be suitable for this?  In addition, we have
> some other questions:
>
> 1.       What is the highest N.A. objective we can use?
>
> 2.       Can we incorporate Bessel beam, or even lattice light sheet
> like Eric Betzig’s group?
>
> 3.       Are there an advantages of using stepper motors for scanning,
> and not galvo-mirrors?  Can galvos be used instead?
>
> Thank you.
>
> Sonny Ly
>
> Staff Scientist
>
> Lawrence Livermore National Laboratory
>
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