[OpenSPIM] changing "objectives"

[Fredrik Ek]

Hi,

We are using a Olympus magnifier 1x,2x and 4x with c-mount together with a Flash4.0 v2 and it works very well with the SPIM setup (20x 0.5 objective).

Best,

Fredrik

Fredrik Ek, PhD,
Researcher, Chemical Biology & Therapeutics
Department of Experimental Medical Science
Lund University, BMC D10
S-221 84 Lund, SWEDEN
------------------------
Fredrik.Ek at med.lu.se<mailto:Fredrik.Ek at med.lu.se>
Phone: +46-727 17 22 05



Från: openspim-bounces at openspim.org [mailto:openspim-bounces at openspim.org] För Feinstein, Timothy N
Skickat: den 22 september 2015 17:35
Till: openspim
Ämne: [OpenSPIM] changing "objectives"

Hi folks,

Thanks to the indispensable help of many users here, our SPIM is up and working.  You are all the best.

Since a SPIM makes it quite hard to change objectives, I bought 1x and a 0.5x C-mount camera adapters to get the equivalent of a 20x and a 10x objective.  It seems to work quite well.  Now I wonder whether anyone has tried a magnifying tube lens like the Olympus U-CA or U-ECA to get a higher power image.  It all depends on whether a 'vanilla' openSPIM design is already imaging near the Nyquist limit.  We get 0.33 um/px using the Olympus 20x/0.5 and a Hamamatsu Flash4.0 v2, and even with deconvolution it may be pointless to magnify the image any further.  Any thoughts appreciated.

Alternatively, has anyone tried keeping a second sample chamber with a higher power objective to swap in as needed?  It seems like a pain but less cumbersome than building a second instrument.

Thanks,


Tim

Timothy Feinstein, Ph.D.
Research Scientist
University of Pittsburgh Department of Developmental Biology


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