[OpenSPIM] changing "objectives"

[Peter Gabriel Pitrone]

Hello Timothy,

We use the U-CA to much success... Aligning the light sheet for each mag is highly recommended. I've even use the 0.5x C-mount with it, but that doesn't work with large chip detectors. Another minor issue I have with it is it's range, it only doubles the mag over 4 slots (Empty, 1.25x, 1.6x, & 2.0x).

Regards,
Pete

Peter Gabriel Pitrone DipRMS
Microscopy and Imaging specialist
in the Dr. Pavel Tomancak group at
the Max Planck Institute of Molecular
Cell Biology and Genetics, Dresden
Pfotenhauerstrasse 108
01307 Dresden
Germany

> On Sep 22, 2015, at 5:34 PM, Feinstein, Timothy N <tnf8 at pitt.edu> wrote:
> 
> Hi folks, 
> 
> Thanks to the indispensable help of many users here, our SPIM is up and working.  You are all the best.  
> 
> Since a SPIM makes it quite hard to change objectives, I bought 1x and a 0.5x C-mount camera adapters to get the equivalent of a 20x and a 10x objective.  It seems to work quite well.  Now I wonder whether anyone has tried a magnifying tube lens like the Olympus U-CA or U-ECA to get a higher power image.  It all depends on whether a 'vanilla' openSPIM design is already imaging near the Nyquist limit.  We get 0.33 um/px using the Olympus 20x/0.5 and a Hamamatsu Flash4.0 v2, and even with deconvolution it may be pointless to magnify the image any further.  Any thoughts appreciated.  
> 
> Alternatively, has anyone tried keeping a second sample chamber with a higher power objective to swap in as needed?  It seems like a pain but less cumbersome than building a second instrument.  
> 
> Thanks, 
> 
> 
> Tim
> 
> Timothy Feinstein, Ph.D. 
> Research Scientist
> University of Pittsburgh Department of Developmental Biology
> 
> 
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