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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Hi,
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">We are using a Olympus magnifier 1x,2x and 4x with c-mount together with a Flash4.0 v2 and it works very well with the SPIM setup (20x 0.5 objective).
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Best,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Fredrik<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black">Fredrik Ek, PhD,
</span><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black">Researcher, Chemical Biology & Therapeutics
</span><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black"><br>
</span><span style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black">Department of Experimental Medical Science
</span><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black"><br>
</span><span style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black">Lund University, BMC D10
</span><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black"><br>
</span><span style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black">S-221 84 Lund, SWEDEN
</span><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black"><br>
</span><span style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black">------------------------
</span><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black"><br>
</span><span lang="SV" style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black"><a href="mailto:Fredrik.Ek@med.lu.se"><span lang="EN-US" style="font-family:"Arial","sans-serif";color:blue">Fredrik.Ek@med.lu.se</span></a></span><span lang="SV" style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black">
</span><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black"><br>
</span><span style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black">Phone: +46-727 17 22 05
</span><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif";color:black"><o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span lang="SV" style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">Från:</span></b><span lang="SV" style="font-size:10.0pt;font-family:"Tahoma","sans-serif""> openspim-bounces@openspim.org [mailto:openspim-bounces@openspim.org]
<b>För </b>Feinstein, Timothy N<br>
<b>Skickat:</b> den 22 september 2015 17:35<br>
<b>Till:</b> openspim<br>
<b>Ämne:</b> [OpenSPIM] changing "objectives"<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Hi folks, <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Thanks to the indispensable help of many users here, our SPIM is up and working. You are all the best. <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Since a SPIM makes it quite hard to change objectives, I bought 1x and a 0.5x C-mount camera adapters to get the equivalent of a 20x and a 10x objective. It
seems to work quite well. Now I wonder whether anyone has tried a magnifying tube lens like the Olympus U-CA or U-ECA to get a higher power image. It all depends on whether a 'vanilla' openSPIM design is already imaging near the Nyquist limit. We get 0.33
um/px using the Olympus 20x/0.5 and a Hamamatsu Flash4.0 v2, and even with deconvolution it may be pointless to magnify the image any further. Any thoughts appreciated. <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Alternatively, has anyone tried keeping a second sample chamber with a higher power objective to swap in as needed? It seems like a pain but less cumbersome
than building a second instrument. <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Thanks, <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Tim<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Timothy Feinstein, Ph.D. <o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">Research Scientist<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black">University of Pittsburgh Department of Developmental Biology<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:10.5pt;font-family:"Calibri","sans-serif";color:black"><o:p> </o:p></span></p>
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