[OpenSPIM] changing "objectives"

[Feinstein, Timothy N]

Hi folks,

Thanks to the indispensable help of many users here, our SPIM is up and working.  You are all the best.

Since a SPIM makes it quite hard to change objectives, I bought 1x and a 0.5x C-mount camera adapters to get the equivalent of a 20x and a 10x objective.  It seems to work quite well.  Now I wonder whether anyone has tried a magnifying tube lens like the Olympus U-CA or U-ECA to get a higher power image.  It all depends on whether a 'vanilla' openSPIM design is already imaging near the Nyquist limit.  We get 0.33 um/px using the Olympus 20x/0.5 and a Hamamatsu Flash4.0 v2, and even with deconvolution it may be pointless to magnify the image any further.  Any thoughts appreciated.

Alternatively, has anyone tried keeping a second sample chamber with a higher power objective to swap in as needed?  It seems like a pain but less cumbersome than building a second instrument.

Thanks,


Tim

Timothy Feinstein, Ph.D.
Research Scientist
University of Pittsburgh Department of Developmental Biology


-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://openspim.org/pipermail/openspim/attachments/20150922/101a91eb/attachment-0002.html>