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<div>Hi folks, </div>
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<div>Thanks to the indispensable help of many users here, our SPIM is up and working. You are all the best. </div>
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<div>Since a SPIM makes it quite hard to change objectives, I bought 1x and a 0.5x C-mount camera adapters to get the equivalent of a 20x and a 10x objective. It seems to work quite well. Now I wonder whether anyone has tried a magnifying tube lens like the
Olympus U-CA or U-ECA to get a higher power image. It all depends on whether a 'vanilla' openSPIM design is already imaging near the Nyquist limit. We get 0.33 um/px using the Olympus 20x/0.5 and a Hamamatsu Flash4.0 v2, and even with deconvolution it may
be pointless to magnify the image any further. Any thoughts appreciated. </div>
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<div>Alternatively, has anyone tried keeping a second sample chamber with a higher power objective to swap in as needed? It seems like a pain but less cumbersome than building a second instrument. </div>
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<div>Thanks, </div>
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<div>Tim</div>
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<div>Timothy Feinstein, Ph.D. </div>
<div>Research Scientist</div>
<div>University of Pittsburgh Department of Developmental Biology</div>
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