<html xmlns:v="urn:schemas-microsoft-com:vml" xmlns:o="urn:schemas-microsoft-com:office:office" xmlns:w="urn:schemas-microsoft-com:office:word" xmlns:m="http://schemas.microsoft.com/office/2004/12/omml" xmlns="http://www.w3.org/TR/REC-html40"><head><meta http-equiv=Content-Type content="text/html; charset=us-ascii"><meta name=Generator content="Microsoft Word 15 (filtered medium)"><style><!--
/* Font Definitions */
@font-face
{font-family:Helvetica;
panose-1:2 11 6 4 2 2 2 2 2 4;}
@font-face
{font-family:"Cambria Math";
panose-1:2 4 5 3 5 4 6 3 2 4;}
@font-face
{font-family:Calibri;
panose-1:2 15 5 2 2 2 4 3 2 4;}
@font-face
{font-family:"Segoe UI";
panose-1:2 11 5 2 4 2 4 2 2 3;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin:0in;
margin-bottom:.0001pt;
font-size:12.0pt;
font-family:"Times New Roman",serif;}
a:link, span.MsoHyperlink
{mso-style-priority:99;
color:blue;
text-decoration:underline;}
a:visited, span.MsoHyperlinkFollowed
{mso-style-priority:99;
color:purple;
text-decoration:underline;}
p.msonormal0, li.msonormal0, div.msonormal0
{mso-style-name:msonormal;
mso-margin-top-alt:auto;
margin-right:0in;
mso-margin-bottom-alt:auto;
margin-left:0in;
font-size:12.0pt;
font-family:"Times New Roman",serif;}
span.apple-tab-span
{mso-style-name:apple-tab-span;}
span.EmailStyle19
{mso-style-type:personal-reply;
font-family:"Calibri",sans-serif;
color:#1F497D;}
.MsoChpDefault
{mso-style-type:export-only;
font-size:10.0pt;}
@page WordSection1
{size:8.5in 11.0in;
margin:1.0in 1.0in 1.0in 1.0in;}
div.WordSection1
{page:WordSection1;}
--></style><!--[if gte mso 9]><xml>
<o:shapedefaults v:ext="edit" spidmax="1026" />
</xml><![endif]--><!--[if gte mso 9]><xml>
<o:shapelayout v:ext="edit">
<o:idmap v:ext="edit" data="1" />
</o:shapelayout></xml><![endif]--></head><body lang=EN-US link=blue vlink=purple><div class=WordSection1><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'>A first version is now in the wiki:<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:10.0pt;font-family:"Segoe UI",sans-serif;color:black'>http://openspim.org/Brightfield</span><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'><o:p></o:p></span></p><p class=MsoNormal><a name="_MailEndCompose"><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'><o:p> </o:p></span></a></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'>Feel free to add your brightfield solutions!<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'>Michael<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:11.0pt;font-family:"Calibri",sans-serif;color:#1F497D'><o:p> </o:p></span></p><div><div style='border:none;border-top:solid #E1E1E1 1.0pt;padding:3.0pt 0in 0in 0in'><p class=MsoNormal><b><span style='font-size:11.0pt;font-family:"Calibri",sans-serif'>From:</span></b><span style='font-size:11.0pt;font-family:"Calibri",sans-serif'> openspim-bounces@openspim.org [mailto:openspim-bounces@openspim.org] <b>On Behalf Of </b>Pavel Tomancak<br><b>Sent:</b> Tuesday, October 13, 2015 8:28 AM<br><b>To:</b> Michael Redd <mredd@cores.utah.edu><br><b>Cc:</b> openspim@openspim.org<br><b>Subject:</b> Re: [OpenSPIM] Cheap Brightfield solution<o:p></o:p></span></p></div></div><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>Hi Michael,<o:p></o:p></p><div><p class=MsoNormal><o:p> </o:p></p></div><div><p class=MsoNormal>Very nice! Would you mind putting it on the wiki?<o:p></o:p></p></div><div><p class=MsoNormal><o:p> </o:p></p></div><div><p class=MsoNormal>All the best<o:p></o:p></p></div><div><p class=MsoNormal><o:p> </o:p></p></div><div><p class=MsoNormal>PAvel<o:p></o:p></p></div><div><p class=MsoNormal><o:p> </o:p></p><div><div><div><div><div><p class=MsoNormal><span style='font-size:9.0pt;font-family:"Helvetica",sans-serif;color:black'>-----------------------------------------------------------------------------------<br>Pavel Tomancak, Ph.D.<br><br>Group Leader<br>Max Planck Institute of Molecular Cell Biology and Genetics<br>Pfotenhauerstr. 108<br>D-01307 Dresden<span class=apple-tab-span> </span>Tel.: +49 351 210 2670<br>Germany<span class=apple-tab-span> </span>Fax: +49 351 210 2020<br>email: <a href="mailto:tomancak@mpi-cbg.de">tomancak@mpi-cbg.de</a><br>homepage: <a href="http://www.mpi-cbg.de/research/research-groups/pavel-tomancak.html">http://www.mpi-cbg.de/research/research-groups/pavel-tomancak.html</a><o:p></o:p></span></p></div><div><p class=MsoNormal><span style='font-size:9.0pt;font-family:"Helvetica",sans-serif;color:black'>twitter: @PavelTomancak<br>-----------------------------------------------------------------------------------<o:p></o:p></span></p></div></div></div></div><p class=MsoNormal style='margin-bottom:12.0pt'><o:p> </o:p></p></div><p class=MsoNormal><o:p> </o:p></p><div><div><p class=MsoNormal>On Oct 12, 2015, at 11:28 PM, Michael Redd <<a href="mailto:mredd@cores.utah.edu">mredd@cores.utah.edu</a>> wrote:<o:p></o:p></p></div><p class=MsoNormal><br><br><o:p></o:p></p><blockquote style='margin-top:5.0pt;margin-bottom:5.0pt'><div><p class=MsoNormal>I just put together a cheap solution for a brightfield light for an openSPIM. It uses a 20 cent green diode, an arduino, a 10K potentiometer, 100 Ohm esistor. The arduino and light are powered by USB and are turned on and off by micro-manager and can be used as a brightfieqld channel in micromanager multi-dimensional acquisition window. The potentiometer and resistor determine the intensity of the light. The diode is fixed in place behind a collimating lens which fills the back aperture of a 10x immersion lens on axis with the sample and imaging lens. <o:p></o:p></p><div><p class=MsoNormal> If your SPIM rig does not have a appropriate port and/or you do not have the 10x lens, one could focus the diode on the sample from above at an oblique angle. This may require a brighter light, but would probably work with the diode I am using. Light level can be dialed in by using different resistors and potentiometers. <o:p></o:p></p></div><div><p class=MsoNormal>I attached photos of the setup and wiring.<o:p></o:p></p></div><div><p class=MsoNormal>If you have any questions don’t hesitate to email me.<o:p></o:p></p></div><div><p class=MsoNormal><o:p> </o:p></p></div><div><p class=MsoNormal>cheers<o:p></o:p></p></div><div><p class=MsoNormal><o:p> </o:p></p><div><p class=MsoNormal><o:p> </o:p></p></div><div><p class=MsoNormal><o:p> </o:p></p></div><div><p class=MsoNormal><o:p> </o:p></p></div><div><p class=MsoNormal>Michael Redd <o:p></o:p></p><div><p class=MsoNormal>Imaging Specialist<br>Cell Imaging Core Facility<br>University of Utah<br>Radiobiology Bldg, Room 48, <br>30 N 2030 E, Salt Lake City, UT 84112<br><br><a href="mailto:michael.redd@hci.utah.edu">michael.redd@hci.utah.edu</a><o:p></o:p></p></div></div></div></div></blockquote></div><p class=MsoNormal><o:p> </o:p></p></div></div></body></html>