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I just put together a cheap solution for a brightfield light for an openSPIM. It uses a 20 cent green diode, an arduino, a 10K potentiometer, 100 Ohm esistor. The arduino and light are powered by USB and are turned on and off by micro-manager and can be used
as a brightfieqld channel in micromanager multi-dimensional acquisition window. The potentiometer and resistor determine the intensity of the light. The diode is fixed in place behind a collimating lens which fills the back aperture of a 10x immersion lens
on axis with the sample and imaging lens.
<div class=""> If your SPIM rig does not have a appropriate port and/or you do not have the 10x lens, one could focus the diode on the sample from above at an oblique angle. This may require a brighter light, but would probably work with the diode I am using.
Light level can be dialed in by using different resistors and potentiometers. </div>
<div class="">I attached photos of the setup and wiring.</div>
<div class="">If you have any questions don’t hesitate to email me.</div>
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<div class="">cheers</div>
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<div class="">Michael Redd
<div apple-content-edited="true" class="">Imaging Specialist<br class="">
Cell Imaging Core Facility<br class="">
University of Utah<br class="">
Radiobiology Bldg, Room 48, <br class="">
30 N 2030 E, Salt Lake City, UT 84112<br class="">
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<a href="mailto:michael.redd@hci.utah.edu" class="">michael.redd@hci.utah.edu</a><br class="">
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<img apple-inline="yes" id="8B372A3E-2E2E-4EC3-801A-AA247A6380DA" height="524" width="874" apple-width="yes" apple-height="yes" src="cid:24D312A2-F29D-437F-8526-CF48F1D269C2@cores.utah.edu" class=""><img apple-inline="yes" id="C7BEEC41-57DC-47C6-8931-60841BEE7091" height="639" width="874" apple-width="yes" apple-height="yes" src="cid:CE1AB7A6-65AD-42EE-B2AE-6E416842776C@cores.utah.edu" class=""><br class="">
<div apple-content-edited="true" class="">Michael Redd<br class="">
Imaging Specialist<br class="">
Cell Imaging Core Facility<br class="">
University of Utah<br class="">
Radiobiology Bldg, Room 48, <br class="">
30 N 2030 E, Salt Lake City, UT 84112<br class="">
<br class="">
<a href="mailto:michael.redd@hci.utah.edu" class="">michael.redd@hci.utah.edu</a><br class="">
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