[OpenSPIM] ID'ing a part

[Jan Huisken]

Hi Neil,

if you already have the 3-axis adjuster, then do not worry about it. I do not think it makes a big difference. I also do not think it is worth buying any fancy mounts that are especially temperature stable. If the mirror is easily accessible it anyway does not hurt to check the alignment before every experiment...

Best
Jan

> On Sep 30, 2015, at 2:34 AM, Anthony, Neil <nantho2 at emory.edu> wrote:
> 
> Hi Jan, I hope the science is treating you well.
>  
> Could I ask about the gimbal vs. the 3-axis adjuster.  How much work is it to remove the tilt using the 3-axis version?  I assume you can achieve a pseudo-gimbal by adjusting a little on two screws at a time…?
>  
> Or maybe, to come at the question from another direction, which would you choose from the two following options?
> -           Polaris mounts all around, but no gimbal (FYI the room temp variation I see is <2°C most days)
> -           Standard mirror mounts but a gimbal at that conjugate plane
>  
> Thanks in advance for your time.
> Neil
>  
>  
> From: openspim-bounces at openspim.org <mailto:openspim-bounces at openspim.org> [mailto:openspim-bounces at openspim.org <mailto:openspim-bounces at openspim.org>] On Behalf Of Jan Huisken
> Sent: Wednesday, August 05, 2015 10:56 AM
> To: openspim at openspim.org <mailto:openspim at openspim.org>
> Subject: Re: [OpenSPIM] ID'ing a part
>  
> Hi Tim
>  
> Correct. This must be a gimbal mirror mount. Sitting in the conjugate plane to the back focal plane you use this mirror to move the light sheet into the focal plane of your detection system (panel C below). If you used a regular (3-screw) mount you would not only translate the sheet but also introduce a tilt (panel D).
>  
> This is also illustrated in Fig. 2 of [Huisken, Jan, and Didier Y R Stainier. 2007. “Even Fluorescence Excitation by Multidirectional Selective Plane Illumination Microscopy (mSPIM).” Optics Letters 32 (17): 2608–10.] 
>  
> Best
> Jan Huisken
>  
>  
> Figure from Weber, Michael, Michaela Mickoleit, and Jan Huisken. 2014. “Light Sheet Microscopy.” Methods in Cell Biology 123: 193–215. doi:10.1016/B978-0-12-420138-5.00011-2.
>  
> <image001.png>
>  
>  
>  
>  
>  
> On Aug 5, 2015, at 4:22 PM, Feinstein, Timothy N <tnf8 at PITT.EDU <mailto:tnf8 at PITT.EDU>> wrote:
>  
> Thanks folks, I got it.  It's a GM100 Ø1" gimbal mirror mount, for adjusting the mirror without changing the axis of rotation.  I can see how a POLARIS in that position can be a little like adjusting cantilever brakes on a bike – lots of axes of adjustment and changing one effects all the others.  Having something there that adjusts like 'V' brakes do (independent axes, less frustration) seems like a good idea.  
>  
> All the best, 
>  
>  
> Tim
>  
> Timothy Feinstein, Ph.D. 
> Research Scientist
> University of Pittsburgh Department of Developmental Biology
>  
>  
> From: <openspim-bounces at openspim.org <mailto:openspim-bounces at openspim.org>> on behalf of Timothy Feinstein <tnf8 at pitt.edu <mailto:tnf8 at pitt.edu>>
> Date: Wednesday, August 5, 2015 at 9:07 AM
> To: "openspim at openspim.org <mailto:openspim at openspim.org>" <openspim at openspim.org <mailto:openspim at openspim.org>>
> Subject: [OpenSPIM] ID'ing a part
>  
> Hello, 
>  
> Please forgive the super-basic question.  In most diagrams on openspim.org <http://openspim.org/> the last mirror housing before the sample chamber is another POLARIS, but in more advanced designs the mirror housing is a larger and unique item not specified in the basic build list.  Can someone let me know what that is?  Many thanks and apologies if this is answered somewhere else, 
>  
>  
> Tim
>  
> Timothy Feinstein, Ph.D. 
> Research Scientist
> University of Pittsburgh Department of Developmental Biology
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