<html><head><meta http-equiv="Content-Type" content="text/html charset=windows-1252"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;" class="">Hi Neil,<div class=""><br class=""></div><div class="">if you already have the 3-axis adjuster, then do not worry about it. I do not think it makes a big difference. I also do not think it is worth buying any fancy mounts that are especially temperature stable. If the mirror is easily accessible it anyway does not hurt to check the alignment before every experiment...</div><div class=""><br class=""></div><div class="">Best</div><div class="">Jan</div><div class=""><br class=""><div><blockquote type="cite" class=""><div class="">On Sep 30, 2015, at 2:34 AM, Anthony, Neil <<a href="mailto:nantho2@emory.edu" class="">nantho2@emory.edu</a>> wrote:</div><br class="Apple-interchange-newline"><div class=""><div class="WordSection1" style="page: WordSection1; font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;"><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">Hi Jan, I hope the science is treating you well.<o:p class=""></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""> </span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">Could I ask about the gimbal vs. the 3-axis adjuster. How much work is it to remove the tilt using the 3-axis version? I assume you can achieve a pseudo-gimbal by adjusting a little on two screws at a time…?<o:p class=""></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""> </span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">Or maybe, to come at the question from another direction, which would you choose from the two following options?<o:p class=""></o:p></span></div><div style="margin: 0in 0in 0.0001pt 20.7pt; font-size: 12pt; font-family: 'Times New Roman', serif; text-indent: -0.25in;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""><span class="">-<span style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-family: 'Times New Roman';" class=""> <span class="Apple-converted-space"> </span></span></span></span><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">Polaris mounts all around, but no gimbal (FYI the room temp variation I see is <2°C most days)<o:p class=""></o:p></span></div><div style="margin: 0in 0in 0.0001pt 20.7pt; font-size: 12pt; font-family: 'Times New Roman', serif; text-indent: -0.25in;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""><span class="">-<span style="font-style: normal; font-variant: normal; font-weight: normal; font-size: 7pt; line-height: normal; font-family: 'Times New Roman';" class=""> <span class="Apple-converted-space"> </span></span></span></span><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">Standard mirror mounts but a gimbal at that conjugate plane<o:p class=""></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""> </span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">Thanks in advance for your time.<o:p class=""></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class="">Neil<o:p class=""></o:p></span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""> </span></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125);" class=""> </span></div><div class=""><div style="border-style: solid none none; border-top-color: rgb(181, 196, 223); border-top-width: 1pt; padding: 3pt 0in 0in;" class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><b class=""><span lang="EN-US" style="font-size: 10pt; font-family: Tahoma, sans-serif;" class="">From:</span></b><span lang="EN-US" style="font-size: 10pt; font-family: Tahoma, sans-serif;" class=""><span class="Apple-converted-space"> </span><a href="mailto:openspim-bounces@openspim.org" style="color: purple; text-decoration: underline;" class="">openspim-bounces@openspim.org</a><span class="Apple-converted-space"> </span>[<a href="mailto:openspim-bounces@openspim.org" style="color: purple; text-decoration: underline;" class="">mailto:openspim-bounces@openspim.org</a>]<span class="Apple-converted-space"> </span><b class="">On Behalf Of<span class="Apple-converted-space"> </span></b>Jan Huisken<br class=""><b class="">Sent:</b><span class="Apple-converted-space"> </span>Wednesday, August 05, 2015 10:56 AM<br class=""><b class="">To:</b><span class="Apple-converted-space"> </span><a href="mailto:openspim@openspim.org" style="color: purple; text-decoration: underline;" class="">openspim@openspim.org</a><br class=""><b class="">Subject:</b><span class="Apple-converted-space"> </span>Re: [OpenSPIM] ID'ing a part<o:p class=""></o:p></span></div></div></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">Hi Tim<o:p class=""></o:p></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">Correct. This must be a gimbal mirror mount. Sitting in the conjugate plane to the back focal plane you use this mirror to move the light sheet into the focal plane of your detection system (panel C below). If you used a regular (3-screw) mount you would not only translate the sheet but also introduce a tilt (panel D).<o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">This is also illustrated in Fig. 2 of [Huisken, Jan, and Didier Y R Stainier. 2007. “Even Fluorescence Excitation by Multidirectional Selective Plane Illumination Microscopy (mSPIM).” Optics Letters 32 (17): 2608–10.] <o:p class=""></o:p></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">Best<o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">Jan Huisken<o:p class=""></o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div></div><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">Figure from Weber, Michael, Michaela Mickoleit, and Jan Huisken. 2014. “Light Sheet Microscopy.” Methods in Cell Biology 123: 193–215. doi:10.1016/B978-0-12-420138-5.00011-2.<o:p class=""></o:p></div></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span id="cid:image001.png@01D0FAF5.7C16E0B0"><image001.png></span><o:p class=""></o:p></div></div><div class=""><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div><div class=""><blockquote style="margin-top: 5pt; margin-bottom: 5pt;" class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">On Aug 5, 2015, at 4:22 PM, Feinstein, Timothy N <<a href="mailto:tnf8@PITT.EDU" style="color: purple; text-decoration: underline;" class="">tnf8@PITT.EDU</a>> wrote:<o:p class=""></o:p></div></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div><div class=""><div class=""><div class=""><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 10.5pt; font-family: Calibri, sans-serif;" class="">Thanks folks, I got it. It's a GM100 Ø1" gimbal mirror mount, for adjusting the mirror without changing the axis of rotation. I can see how a POLARIS in that position can be a little like adjusting cantilever brakes on a bike – lots of axes of adjustment and changing one effects all the others. Having something there that adjusts like 'V' brakes do (independent axes, less frustration) seems like a good idea. <o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 10.5pt; font-family: Calibri, sans-serif;" class=""> </span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 10.5pt; font-family: Calibri, sans-serif;" class="">All the best, <o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 10.5pt; font-family: Calibri, sans-serif;" class=""> </span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 10.5pt; font-family: Calibri, sans-serif;" class=""> </span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 10.5pt; font-family: Calibri, sans-serif;" class="">Tim<o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 10.5pt; font-family: Calibri, sans-serif;" class=""> </span></div></div><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 10.5pt; font-family: Calibri, sans-serif;" class="">Timothy Feinstein, Ph.D. <o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 10.5pt; font-family: Calibri, sans-serif;" class="">Research Scientist<o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 10.5pt; font-family: Calibri, sans-serif;" class="">University of Pittsburgh Department of Developmental Biology<o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 10.5pt; font-family: Calibri, sans-serif;" class=""> </span></div></div></div></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 10.5pt; font-family: Calibri, sans-serif;" class=""> </span></div></div><div style="border-style: solid none none; border-top-color: rgb(181, 196, 223); border-top-width: 1pt; padding: 3pt 0in 0in;" class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><b class=""><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class="">From:<span class="Apple-converted-space"> </span></span></b><span style="font-size: 11pt; font-family: Calibri, sans-serif;" class=""><<a href="mailto:openspim-bounces@openspim.org" style="color: purple; text-decoration: underline;" class="">openspim-bounces@openspim.org</a>> on behalf of Timothy Feinstein <<a href="mailto:tnf8@pitt.edu" style="color: purple; text-decoration: underline;" class="">tnf8@pitt.edu</a>><br class=""><b class="">Date:<span class="Apple-converted-space"> </span></b>Wednesday, August 5, 2015 at 9:07 AM<br class=""><b class="">To:<span class="Apple-converted-space"> </span></b>"<a href="mailto:openspim@openspim.org" style="color: purple; text-decoration: underline;" class="">openspim@openspim.org</a>" <<a href="mailto:openspim@openspim.org" style="color: purple; text-decoration: underline;" class="">openspim@openspim.org</a>><br class=""><b class="">Subject:<span class="Apple-converted-space"> </span></b>[OpenSPIM] ID'ing a part<o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 10.5pt; font-family: Calibri, sans-serif;" class=""> </span></div></div><div class=""><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 10.5pt; font-family: Calibri, sans-serif;" class="">Hello, <o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 10.5pt; font-family: Calibri, sans-serif;" class=""> </span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 10.5pt; font-family: Calibri, sans-serif;" class="">Please forgive the super-basic question. In most diagrams on<span class="Apple-converted-space"> </span><a href="http://openspim.org/" style="color: purple; text-decoration: underline;" class="">openspim.org</a><span class="Apple-converted-space"> </span>the last mirror housing before the sample chamber is another POLARIS, but in more advanced designs the mirror housing is a larger and unique item not specified in the basic build list. Can someone let me know what that is? Many thanks and apologies if this is answered somewhere else, <o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 10.5pt; font-family: Calibri, sans-serif;" class=""> </span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 10.5pt; font-family: Calibri, sans-serif;" class=""> </span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 10.5pt; font-family: Calibri, sans-serif;" class="">Tim<o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 10.5pt; font-family: Calibri, sans-serif;" class=""> </span></div></div><div class=""><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 10.5pt; font-family: Calibri, sans-serif;" class="">Timothy Feinstein, Ph.D. <o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 10.5pt; font-family: Calibri, sans-serif;" class="">Research Scientist<o:p class=""></o:p></span></div></div><div class=""><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><span style="font-size: 10.5pt; font-family: Calibri, sans-serif;" class="">University of Pittsburgh Department of Developmental Biology<o:p class=""></o:p></span></div></div></div></div></div></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class="">_______________________________________________<br class="">OpenSPIM mailing list<br class=""><a href="mailto:OpenSPIM@openspim.org" style="color: purple; text-decoration: underline;" class="">OpenSPIM@openspim.org</a><br class=""><a href="http://openspim.org/mailman/listinfo/openspim" style="color: purple; text-decoration: underline;" class="">http://openspim.org/mailman/listinfo/openspim</a><o:p class=""></o:p></div></div></blockquote></div><div style="margin: 0in 0in 0.0001pt; font-size: 12pt; font-family: 'Times New Roman', serif;" class=""><o:p class=""> </o:p></div></div></div></div></div><br style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><hr style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><font face="Arial" color="Gray" size="1" style="font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px;" class=""><br class="">This e-mail message (including any attachments) is for the sole use of<br class="">the intended recipient(s) and may contain confidential and privileged<br class="">information. If the reader of this message is not the intended<br class="">recipient, you are hereby notified that any dissemination, distribution<br class="">or copying of this message (including any attachments) is strictly<br class="">prohibited.<br class=""><br class="">If you have received this message in error, please contact<br class="">the sender by reply e-mail message and destroy all copies of the<br class="">original message (including attachments).<br class=""></font><span style="font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: auto; text-align: start; text-indent: 0px; text-transform: none; white-space: normal; widows: auto; word-spacing: 0px; -webkit-text-stroke-width: 0px; float: none; display: inline !important;" class=""></span></div></blockquote></div><br class=""></div></body></html>