[OpenSPIM] max acquisition depth

[Martin Capek]

Dear OpenSPIM users,
I would have a question: In "Stack at this Z plus" it is possible to set 
maximum of 1500 microns. Is it possible to circumvent somehow this 
limitation? We need to acquire embryonic mouse hearts with greater 
diameter, thus having possibility to capture specimens up to 2.5-3 mm in 
depth would be useful. At the moment I acquire two consecutive stacks of 
optical sections for one xy position, but differing in z. Afterwards I 
stitch them together.
Moreover, having some tool that would be able to capture automatically a 
matrix of overlapping fields of view in xy with the same depth settings 
would be superior. I know, it is not easy to program. I am just 
describing what would be useful for us, since we work with relatively 
big pieces of fixed specimens, up to 4x4x4mm cubic.
Thank you very much for your help in advance!
Best regards,
Martin

----------------------

Martin Capek, Ph.D.
Dpt. Biomathematics

Institute of Physiology ASCR, v.v.i.
Videnska 1083
142 20 Praha 4

Tel. +420 241 062 334
http://fgu.cas.cz <http://fgu.cas.cz/>


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